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Immunohistochemistry and Immunofluorescence
2022Immunohistochemistry (IHC) is one of the most widely used protein detection techniques. The principle of this technique is based on the binding of a specific antibody to a matching specific antigen in tissue. The bound antigen-antibody complex then is visualized using a range of detection techniques.
Haizal Mohd, Hussaini +2 more
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Optimizing Direct Immunofluorescence
2014Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes.
Ian D, Odell, Deborah, Cook
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Indirect immunofluorescence microscopy
2006This is a standard protocol for the detection of intracellular proteins by indirect immunofluorescence microscopy in DT40. It has been used extensively to investigate the intracellular distribution of various proteins of the endocytic machinery.
Frank R, Wettey, Antony P, Jackson
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Multiplex Immunofluorescence Assays
2019Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue section emerged in the last years as very powerful tools to study tumor immune contexture. These revolutionary technologies are providing a deep methodology for tumor evaluation in formalin-fixed and paraffin-embedded (FFPE) to improve the understanding of tumor
Alejandro, Francisco-Cruz +3 more
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Standardization In Immunofluorescence
Annals of the New York Academy of Sciences, 1982E J, Holborow, G D, Johnson
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