Results 251 to 260 of about 853,198 (295)
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Current Protocols in Molecular Biology, 2008
AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
Marsha, Goldstein, Simon, Watkins
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AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
Marsha, Goldstein, Simon, Watkins
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Fluorescent Immunohistochemistry
2021Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry. However, analysis of
Vanessa, Moreno +2 more
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Immunohistochemistry and Immunofluorescence
2022Immunohistochemistry (IHC) is one of the most widely used protein detection techniques. The principle of this technique is based on the binding of a specific antibody to a matching specific antigen in tissue. The bound antigen-antibody complex then is visualized using a range of detection techniques.
Haizal Mohd, Hussaini +2 more
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Immunohistochemistry Microarrays
Analytical Chemistry, 2017Immunohistochemistry (IHC) on tissue sections is widely used for quantifying the expression patterns of proteins and is part of the standard of care for cancer diagnosis and prognosis, but is limited to staining a single protein per tissue. Tissue microarray and microfluidics staining methods have emerged as powerful high throughput techniques, but ...
Huiyan Li +5 more
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, 2019 enograft tumor from in vivo experiment was havested and fixed in 4% paraformaldehyde. Paraffin-embedded sections were used for haematoxylin-eosin (HE) staining and immunohistochemistry assays. For immunohistochemical staining, the sections were incubated
Pai Pang (5361815) +10 more
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Immunohistochemistry of retinoblastomas
Journal of Neuro-Oncology, 1987Immunohistochemical stains using neuronal and glial marker proteins were applied to retinoblastomas tissues from 14 children. Among the neurofilament triplet proteins, NF68Kd positive cells were observed in 12. Few NF160Kd positive cells were noted in 2, and NF210Kd positive cells were not detected.
H, Sawa +4 more
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Immunohistochemistry of Otosclerosis
Acta Oto-Laryngologica, 1990Immunoglobulins G (IgG) and A (IgA) were found in plasma cells, osteocytes, chondrocytes and in connective tissue of active otosclerotic (= otospongiotic) lesions. The application of antibodies against paramyxovirus and rubella virus antigens reproducibly determined the expression of these antigens at different sites in the otosclerotic stapes, but ...
W, Arnold, I, Friedmann
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Current Protocols in Cytometry, 1995
AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
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AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy.
openaire +2 more sources
Current Protocols in Immunology, 2002
AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or from suspension or adherent cultures, or fixed ...
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AbstractThis unit describes several methods for localizing specific antigens in various tissue and cell preparations using immunohistochemistry (IHC). Protocols describe preparation of suitable material for IHC including fresh, unfixed, frozen tissue specimens; unfixed cells, either freshly isolated or from suspension or adherent cultures, or fixed ...
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Microwaves for immunohistochemistry
Micron, 1994Microwaves are now widely used in immunohistochemistry for fixing and stabilizing tissue prior to embedding and cutting, for antigen retrieval and for immunoincubations. These techniques can be used for frozen sections and for material embedded in paraffin and plastic. Material prepared in this way shows high contrast in light microscopy. In principle,
M E, Boon, L P, Kok
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