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2022
This chapter begins by outlining the principles of the immune system and the fundamentals of immunoassay, which are essential to the understanding of routine immunological diagnostic assays. Immunological techniques largely depend upon the visualization of antibody–antigen reactions, detecting the formation of immune complexes or the attachment of ...
Christine Yates, Qiuyu Wang
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This chapter begins by outlining the principles of the immune system and the fundamentals of immunoassay, which are essential to the understanding of routine immunological diagnostic assays. Immunological techniques largely depend upon the visualization of antibody–antigen reactions, detecting the formation of immune complexes or the attachment of ...
Christine Yates, Qiuyu Wang
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Immunological techniques in biotechnology research
Biochemical Society Transactions, 1992Specific interactions between antigen and antibody provided the basis for a variety of applications of monoclonal antibodies of mouse origin. In enzymelinked immunoassays (e.1.is.a.) they are used for quantification and qualification of recombinant DNA-derived proteins and contaminants of proteinaceous nature. In addition, in epitope mapping they are a
R G, Werner +4 more
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Spontaneous Fibrinolysis Demonstrated by Immunological Technique
Thrombosis and Haemostasis, 1967SummaryThe occurrence of spontaneous fibrinolysis has been investigated in various medical diseases, e. g. myocardial infarction, cerebral stroke, phlebothrombosis, pulmonary embolism, metastasing cancer, and others. Serum from patients has been tested for fibrinolytic break-down products by use of immunodiffusion and Immunoelectrophoresis with double ...
B, Laursen, J, Gormsen
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Immunological Technique for Protein Isolation
Science, 1960Carefully coagulated antibody or antigen-antibody complexes may be used as specific adsorbents for antigen, and the antigen may be released subsequently by increasing the acidity. No cross adsorption appears to take place. The procedure may prove useful for the isolation of tissue-specific proteins (including those in disease states), toxins, or ...
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Analytic Immunologic Techniques
1988Analytic immunologic procedures have become important parts of the arsenal of techniques for describing and elucidating physiologic and developmental changes in naturally occurring antigens (Ags) from insects. Antisera, which are solutions of the natural defense secretions produced by vertebrate immune systems in response to foreign antigens, can be ...
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Immunology Laboratory Techniques
2002This chapter aims to assist readers in understanding the basic principles of commonly applied diagnostic tests in allergic and immunologic diseases. Recent advances in the technology and availability of highly specific immunologic reagents have lessened the burden of the diagnosis and management of patients with allergic and immunologic diseases.
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Detection of Phosphorylation by Immunological Techniques
Current Protocols in Protein Science, 1995AbstractPhosphorylation of unlabeled proteins can be detected using immunological or enzymatic techniques. Anti‐phosphotyrosine antibodies are used with immunoblots to detect tyrosine phosphorylation. This unit presents a protocol employing anti‐phosphotyrosine antibodies with detection by either 125I‐labeled protein A or enhanced chemiluminescence ...
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Review of Immunologic Techniques
1984Immunologic methods provide highly specific and unique tools to determine minute amounts of antigenic proteins in serum, plasma, and other biologic fluids. Such methods of protein detection are called immunoassays. In general, immunoassays are classified as type I and type II immunoassays depending upon the quantitative use of antibody.
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