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Immunological techniques in biotechnology research
Biochemical Society Transactions, 1992Specific interactions between antigen and antibody provided the basis for a variety of applications of monoclonal antibodies of mouse origin. In enzymelinked immunoassays (e.1.is.a.) they are used for quantification and qualification of recombinant DNA-derived proteins and contaminants of proteinaceous nature. In addition, in epitope mapping they are a
R G, Werner +4 more
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Immunological techniques in viral hepatitis
Journal of Hepatology, 2007The need to quantitate and monitor immune responses of large patient cohorts with standardized techniques is increasing due to the growing range of treatment options for hepatitis B and hepatitis C, the development of combination therapies, and candidate experimental vaccines for HCV.
Barbara, Rehermann, Nikolai V, Naoumov
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Immunological Techniques in and for Developing Countries
Scandinavian Journal of Immunology, 1992A review is given of the problems and challenges in using immunological techniques in and for developing countries.
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Detection of Phosphorylation by Immunological Techniques
Current Protocols in Protein Science, 1995AbstractPhosphorylation of unlabeled proteins can be detected using immunological or enzymatic techniques. Anti‐phosphotyrosine antibodies are used with immunoblots to detect tyrosine phosphorylation. This unit presents a protocol employing anti‐phosphotyrosine antibodies with detection by either 125I‐labeled protein A or enhanced chemiluminescence ...
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Spontaneous Fibrinolysis Demonstrated by Immunological Technique
Thrombosis and Haemostasis, 1967SummaryThe occurrence of spontaneous fibrinolysis has been investigated in various medical diseases, e. g. myocardial infarction, cerebral stroke, phlebothrombosis, pulmonary embolism, metastasing cancer, and others. Serum from patients has been tested for fibrinolytic break-down products by use of immunodiffusion and Immunoelectrophoresis with double ...
B, Laursen, J, Gormsen
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Immunological Technique for Protein Isolation
Science, 1960Carefully coagulated antibody or antigen-antibody complexes may be used as specific adsorbents for antigen, and the antigen may be released subsequently by increasing the acidity. No cross adsorption appears to take place. The procedure may prove useful for the isolation of tissue-specific proteins (including those in disease states), toxins, or ...
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Selected biochemical techniques as applied to immunology
Current Opinion in Immunology, 1989As an undergraduate back in 1964, I recall naively suggesting to my organic chemistry professor that he should use antibodies to solve the problem of resolving racemic mixtures, His reply was that, even if antisera with such kne specificity could be found, I should reject me idea because it was not ‘real chemistry’. Twenty-live years later, in 1989, it
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Experimental Dermatitis by Means of the Immunological Technique
Acta Oto-Laryngologica, 1988Animal experiments using rabbits were performed to explore the relationship between the tonsils and pustulosis palmaris et plantaris. Since in the abscess of the pustulosis palmaris et plantaris many pleomorphic leukocytes are seen histologically, sensitized mononuclear cells (MNC) isolated from sensitized rabbits were transferred intravenously in ...
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Platelet Aggregation as a Quantitative Immunologic Technique
Immunological Communications, 1973A rapid quantitative immunologic method based on the sensitivity of platelets to immune complexes is described. Platelet-rich-plasma (PRP) from sensitized animals exposed to various concentrations of antigen (or antigen t anti-serum) is sedimented.
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