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Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline
Bioscientia Medicina : Journal of Biomedicine and Translational Research, 2021 ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where
Rachmat Hidayat, Patricia Wulandari
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Hemadsorption immunosorbent technique for the detection of dengue immunoglobulin M antibody [PDF]
Journal of Clinical Microbiology, 1986 We developed a highly specific, sensitive, and economical hemadsorption immunosorbent technique for the detection of dengue-specific immunoglobulin M (IgM) antibody. The technique is based on the reaction of human sera with anti-human IgM immobilized onto a solid phase followed by the detection of dengue-specific IgM by the addition of a known quantity
K Gunasegaran +5 more
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Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody [PDF]
Journal of Clinical Microbiology, 1982 We used hemadsorption immunosorbent technique (HIT) to detect mumps immunoglobulin M (IgM) antibody. IgM from human sera was adsorbed into anti-human IgM-coated wells in plates, and mumps-specific IgM was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively.
J T, van der Logt +3 more
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Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research [PDF]
Journal of Hygiene, 1978 SummaryThe indirect ELISA technique has been developed successfully to measure antibodies to foot-and-mouth disease virus (FMDV) in cattle sera. Preliminary studies using a standard serum assay show that reproducible results are obtained. The method should prove useful for the examination of antibody titres in sera from large numbers of cattle or other
J. R. Crowther, E. M. E. Abu Elzein
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Clinical Chemistry, 1973 Abstract A commercial kit for determining immunoreactive insulin, the "Phadebas Insulin Test," was methodically evaluated to establish the optimal conditions for this immunoassay. Sensitivity, day-to-day and within-run precision, recovery, accuracy, and specificity are excellent. Results by this method compare favorably with those of the
Carlos A. Velasco +2 more
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Journal of General Virology, 1979 The rubella haemagglutination inhibition (HAI) test has been modified for the detection of rubella-specific IgM. The rubella HAI test is performed in microtitre plate wells in which IgM from patients' sera has been selectively retained by anti-human IgM bound to the polystyrene surface. The test requires only anti-human IgM-coated microtitre plates, in
J. A. Wilhelm, U. Krech
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Development of biomimetic enzyme-linked immunosorbent assay based on molecular imprinting technique for semicarbazide detection [PDF]
Food and Agricultural Immunology, 2019 Herein, we present a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of semicarbazide (SEM) using a molecularly imprinted polymer (MIP) film as the bionic antibody. The best monomer (methacrylic acid) and molar ratio of monomer to template (4:1) were determined with the aid of computational ...
Xianghong Wang +5 more
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Disk-based enzyme-linked immunosorbent assays using the liquid-aliquoting and siphoning-evacuation technique [PDF]
Biomicrofluidics, 2018 A cost-effective way to carry out multiple enzyme-linked immunosorbent assays (ELISAs) on a centrifugal platform using the liquid-aliquoting and siphoning-evacuation (LASE) technique was developed in this paper. Instead of preloading all the reagents in the reservoirs before testing, each reagent was loaded only one time during testing. The reagent was
Ho-Chin Wu, Yen-Hao Chen, Chih-Hsin Shih
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Journal of General Virology, 1979 Enzyme labelled immunosorbent assays (ELISA) have been developed to detect and quantify foot and mouth disease (FMD) virus using flexible plastic microtitre plates. The methods were successful for the specific detection of FMD virus and were 50 to 100 times more sensitive than the complement fixation test. The application of the ELISA techniques to FMD
J. R. Crowther, E. M. E. Abu-El Zein
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Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection [PDF]
Journal of Clinical Microbiology, 1986 A comparative study of herpes simplex virus diagnosis by standard cell culture and a new hybrid test (enzyme-linked immunosorbent assay spin amplification technique) was done on 300 specimens. The new test was found to be equally sensitive and specific, much less expensive to perform, and to report all results in 48 h.
J Vallabhaneni +5 more
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