High-performance liquid chromatography and Enzyme-Linked Immunosorbent Assay techniques for detection and quantification of aflatoxin B1 in feed samples: a comparative study [PDF]
BMC Research Notes, 2019 Objective Comparison was done between high-performance liquid chromatography (HPLC) and a competitive enzyme-linked immunosorbent assay (ELISA) for detection and quantification of aflatoxin B1 (AFB1) in feed samples.
Achenef Melaku Beyene +4 more
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Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline
Bioscientia Medicina : Journal of Biomedicine and Translational Research, 2021 ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where
Rachmat Hidayat, null Patricia Wulandari
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Validation of HPLC and Enzyme-Linked Immunosorbent Assay (ELISA) Techniques for Detection and Quantification of Aflatoxins in Different Food Samples [PDF]
Foods, 2020 Background: In Jordan as in other worldwide countries, mycotoxins are considered a serious national problem in food supplies. As a result, almost all nations are setting and adopting different regulations targeting the control of mycotoxins levels in the
Sharaf S. Omar +2 more
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Hemadsorption immunosorbent technique for determination of rubella immunoglobulin M antibody [PDF]
Journal of Clinical Microbiology, 1981 A highly specific and sensitive hemadsorption immunosorbent technique for measuring rubella immunoglobulin M (IgM) antibody is described. IgM from human sera was absorbed into anti-human IgM-coated wells in plates and rubella-specific IgM was detected by adding rubella virus hemagglutinin and a small quantity of sheep erythrocytes.
J T, van der Logt +2 more
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Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection [PDF]
Journal of Clinical Microbiology, 1986 A comparative study of herpes simplex virus diagnosis by standard cell culture and a new hybrid test (enzyme-linked immunosorbent assay spin amplification technique) was done on 300 specimens. The new test was found to be equally sensitive and specific, much less expensive to perform, and to report all results in 48 h.
F J, Michalski +5 more
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Sequential use of real-time polymerase chain reaction and enzyme-linked immunosorbent assay techniques verifies adulteration of fermented sausages with chicken meat [PDF]
Animal Bioscience, 2021 Objective Detection of adulteration in processed meats is an important issue for some countries due to substitution of beef with a cheaper source of protein like poultry.
Hakan Benli, Elif Barutcu
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Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody [PDF]
Journal of Clinical Microbiology, 1982 We used hemadsorption immunosorbent technique (HIT) to detect mumps immunoglobulin M (IgM) antibody. IgM from human sera was adsorbed into anti-human IgM-coated wells in plates, and mumps-specific IgM was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively.
J T, van der Logt +3 more
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Microfluidic preparation of size- and lamellarity-controlled liposomes using a static mixer and the advantage of a multilamellar structure in biosensing applications [PDF]
Frontiers in Bioengineering and BiotechnologyBackgroundLiposomes are useful tools for amplifying signals in biosensing techniques, which are used to detect and analyze biological molecules. The goal of this study is to improve the sensitivity of immunosorbent assays by optimizing the self-assembled
Makoto Kiuchi +3 more
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Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research [PDF]
Journal of Hygiene, 1978 SummaryThe indirect ELISA technique has been developed successfully to measure antibodies to foot-and-mouth disease virus (FMDV) in cattle sera. Preliminary studies using a standard serum assay show that reproducible results are obtained. The method should prove useful for the examination of antibody titres in sera from large numbers of cattle or other
E M, Abu Elzein, J R, Crowther
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A simple washing technique for solid-phase radioimmunoassays and enzyme-linked immunosorbent assays. [PDF]
Journal of Clinical Pathology, 1981 H Park
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