Results 321 to 330 of about 365,054 (347)
Application of the Partial Least Square Technique to Identify Critical Variables in the Immunosorbent Manufacturing [PDF]
Chromatographia, 2008 The partial least square technique (PLS) was applied to the monoclonal antibody (Mab) CB.Hep-1 immunosorbent manufacturing to determine the influence of cyanate ester concentration, ligand concentration and target ligand density on Mab coupling efficiency, elution capacity, Hepatitis B surface antigen purity and ligand leakage (output variables ...
Kenia M. Sánchez +16 more
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Techniques and instrumentation for preparative immunosorbent separations
Journal of Chromatography A, 1978Abstract Studies of the physical and chemical characteristics of immunosorbents have allowed operational improvements to be made in their application to preparative separation of pure antigens and monospecific antibodies. The loss of immunochemical reactivity with increasing protein substitution and the limitations imposed by the porous nature of ...
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Enzyme-linked immunosorbent assay (ELISA) for HIV antibody by a glass slide technique
Journal of Immunological Methods, 1991An enzyme-linked immunosorbent assay (ELISA) technique is described which utilizes a commercially available glass microscope slide coated with hydrophobic teflon in such a pattern as to give 30 small circular wells, each of which has a glass bottom.
M. Jane Ludwig +3 more
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Vox Sanguinis, 1983
Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
J. T. Holt +5 more
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Abstract. A solid‐phase kinetic enzyme‐linked immunosorbent assay has been developed to measure binding of antibodies to purified or synthetic blood group antigens and tested in the Lewis blood group system. Chemically synthesized Lewis a antigen is used as the target for the binding of serum antibody in this sandwich‐type assay.
J. T. Holt +5 more
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The Analyst, 2014
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information ...
Zhang, Shiyun +3 more
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Journal of Immunological Methods, 1994
This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
H. Sénéchal +6 more
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This study describes the use of electrophoretically purified antigens blotted onto nitrocellulose, as solid phase antigens for enzyme-linked immunosorbent assays. This procedure is called DMISA, for dissociated membrane immunosorbent assay. The method is illustrated using immunoblotted antigens of Dactylis glomerata grass pollen extract.
H. Sénéchal +6 more
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2020
Inflammation is part of a defense reaction of live tissues that is triggered by pathogens, chemical reagents, trauma, and radiation. Understanding the inflammatory process triggered by Zika virus (ZIKV) is important to better understand the pathogen-host interaction.
Raquel das Neves Almeida +3 more
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Inflammation is part of a defense reaction of live tissues that is triggered by pathogens, chemical reagents, trauma, and radiation. Understanding the inflammatory process triggered by Zika virus (ZIKV) is important to better understand the pathogen-host interaction.
Raquel das Neves Almeida +3 more
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American Journal of Veterinary Research, 1985
SUMMARY A microenzyme-linked immunosorbent assay (dot-elisa) was modified for making an immunodiagnosis of Fasciola hepatica infections in sheep. Sheep were alloted as follows: group I—3 controls and 4 principals, each inoculated with 500 metacercariae; group II—3 controls and 7 principals, each inoculated with 250 metacercariae; and group III—3 ...
G L, Zimmerman, M J, Nelson, C R, Clark
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SUMMARY A microenzyme-linked immunosorbent assay (dot-elisa) was modified for making an immunodiagnosis of Fasciola hepatica infections in sheep. Sheep were alloted as follows: group I—3 controls and 4 principals, each inoculated with 500 metacercariae; group II—3 controls and 7 principals, each inoculated with 250 metacercariae; and group III—3 ...
G L, Zimmerman, M J, Nelson, C R, Clark
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American Journal of Veterinary Research, 1986
SUMMARY A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs.
S, Bernard +3 more
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SUMMARY A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs.
S, Bernard +3 more
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The Journal of laboratory and clinical medicine, 1965
Abstract A specific inhibitor of PTC from the plasma of a PTC-deficient patient was partially purified. The inhibitor was made insoluble by chemically binding it to cellulose; this made it possible to add the same portion of inhibitor cellulose repeatedly to fresh aliquots of plasma.
Harold R. Roberts +2 more
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Abstract A specific inhibitor of PTC from the plasma of a PTC-deficient patient was partially purified. The inhibitor was made insoluble by chemically binding it to cellulose; this made it possible to add the same portion of inhibitor cellulose repeatedly to fresh aliquots of plasma.
Harold R. Roberts +2 more
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