Results 331 to 340 of about 365,054 (347)
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Journal of Chemical Education, 2012
ELISA (enzyme-linked immunosorbent assay) is a widely used technique with applications in disease diagnosis, detection of contaminated foods, and screening for drugs of abuse or environmental contaminants. However, published protocols with a focus on quantitative detection of small molecules designed for teaching laboratories are limited. A competitive,
Powers, Jennifer L. +5 more
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ELISA (enzyme-linked immunosorbent assay) is a widely used technique with applications in disease diagnosis, detection of contaminated foods, and screening for drugs of abuse or environmental contaminants. However, published protocols with a focus on quantitative detection of small molecules designed for teaching laboratories are limited. A competitive,
Powers, Jennifer L. +5 more
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1987
New techniques are described to improve the sensitivity and specificity of detection and isolation procedures for bacteria in epidemiological and quality control studies.
M. A. Ruissen +2 more
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New techniques are described to improve the sensitivity and specificity of detection and isolation procedures for bacteria in epidemiological and quality control studies.
M. A. Ruissen +2 more
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Revue Scientifique et Technique de l'OIE, 1993
Enzyme-linked immunosorbent assay (ELISA) techniques for the detection of antibodies are now widely used throughout the world for the diagnosis of infectious diseases in veterinary medicine. Although many laboratories have independently developed ELISA techniques for their own purposes, little progress has been made with respect to the international ...
E.M.A. van Rooij +4 more
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Enzyme-linked immunosorbent assay (ELISA) techniques for the detection of antibodies are now widely used throughout the world for the diagnosis of infectious diseases in veterinary medicine. Although many laboratories have independently developed ELISA techniques for their own purposes, little progress has been made with respect to the international ...
E.M.A. van Rooij +4 more
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Journal of Infectious Diseases, 1983
Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay variation.
Robert C. Radin +5 more
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Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay variation.
Robert C. Radin +5 more
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Preventive Veterinary Medicine, 1998
Approximately 0.2% (n = 4397) of the bovids (cattle and buffalo) in Sri Lanka were sampled, from June 1992 using a multi-stage sampling procedure. Serum antibodies for the rinderpest virus were detected using the competitive enzyme-linked immunosorbent assay.
Indira Silva +2 more
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Approximately 0.2% (n = 4397) of the bovids (cattle and buffalo) in Sri Lanka were sampled, from June 1992 using a multi-stage sampling procedure. Serum antibodies for the rinderpest virus were detected using the competitive enzyme-linked immunosorbent assay.
Indira Silva +2 more
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Analytical Biochemistry, 1991
PCR products obtained using primers carrying at their 5' ends biotin and an antigenic group (e.g., fluorescein) can be quantitatively analyzed by immunological techniques. The procedure described here does not require electrophoretic separation and/or hybridization with radioactive probes.
Axel Landgraf +2 more
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PCR products obtained using primers carrying at their 5' ends biotin and an antigenic group (e.g., fluorescein) can be quantitatively analyzed by immunological techniques. The procedure described here does not require electrophoretic separation and/or hybridization with radioactive probes.
Axel Landgraf +2 more
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Drug Testing and Analysis
AbstractELISA assays are commonly used for drug screening by racing laboratories but are known to suffer from limited specificity. Inaccurate ELISA screening results are typically produced by non‐specific antibody interactions or by the retention of chromogenic material in the sample well due to sample degradation.
Rohan Steel, Antony Botteon, Mark Timms
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AbstractELISA assays are commonly used for drug screening by racing laboratories but are known to suffer from limited specificity. Inaccurate ELISA screening results are typically produced by non‐specific antibody interactions or by the retention of chromogenic material in the sample well due to sample degradation.
Rohan Steel, Antony Botteon, Mark Timms
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Journal of Immunological Methods, 1983
A micro enzyme-linked immunosorbent assay utilizing antigen dotted onto nitrocellulose filter discs (Dot-ELISA) was developed for the rapid diagnosis of visceral leishmaniasis. Leishmania donovani promastigotes applied to filter discs in volumes of 1 microliter were placed in 96-well microtiter plates, blocked with bovine serum albumin, then incubated ...
Michael G. Pappas +2 more
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A micro enzyme-linked immunosorbent assay utilizing antigen dotted onto nitrocellulose filter discs (Dot-ELISA) was developed for the rapid diagnosis of visceral leishmaniasis. Leishmania donovani promastigotes applied to filter discs in volumes of 1 microliter were placed in 96-well microtiter plates, blocked with bovine serum albumin, then incubated ...
Michael G. Pappas +2 more
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Toxicon, 1983
The development and application of immunoassay techniques in relation to snake venom research is reviewed. Enzyme linked immunosorbent assay (ELISA) is compared with radioimmunoassay, immunodiffusion, immunofluorescence, haemagglutination and immunoelectrophoresis. It is concluded that ELISA is the most versatile immunoassay technique so far applied to
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The development and application of immunoassay techniques in relation to snake venom research is reviewed. Enzyme linked immunosorbent assay (ELISA) is compared with radioimmunoassay, immunodiffusion, immunofluorescence, haemagglutination and immunoelectrophoresis. It is concluded that ELISA is the most versatile immunoassay technique so far applied to
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The enzyme-linked immunosorbent assay (ELISA) (theory, technique and applications).
La Ricerca in clinica e in laboratorio, 1979Enzyme-linked immunosorbent assays (ELISA) are useful for measuring levels on antigens and antibodies. Both competitive and sandwich methods can be used. These assays are analogous to radioimmunoassays in format and have similar sensitivity. However, results can sometimes by read visually and even for objective readings only simple equipment is needed.
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