Results 221 to 230 of about 128,845 (282)

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

A Simplified Analytical Model for Strip Buckling in the Pressure-Assisted Milling Process. [PDF]

Materials (Basel)
Wang X, Chen K, Lin Y, He Z.
europepmc   +1 more source

Early Radial Extracorporeal Shockwave Stimulation on Proximal Tibial Circular Osteotomy Site Enhanced Heterotopic Skin Wound Healing via Small Extracellular Vesicles

Advanced Science, EarlyView.
A schematic diagram of the mechanism where small extracellular vesicles mediate the enhancement of heterotopic wound healing by radial extracorporeal shockwave stimulation at a proximal tibial osteotomy site. ABSTRACT Wound healing represents a complex biological process necessitating innovative therapeutic approaches, particularly for chronic wounds ...
Xiaoping Xie, Songqi Bi, Bang Su, Rongqi Zhou, Xudong Li, Zhixing Yan, Xiaoyang Zhou, Fanxu Wang, Tiecheng Yu   +8 more
wiley   +1 more source

A Study of Imperfect Cylindrical Steel Tubes under Global Bending and Varying Support Conditions [PDF]

, 2015
Fajuyitan, OK, Rotter, JM, Sadowski, AJ
core  

Controlling colloidal flow through a microfluidic Y-junction. [PDF]

Commun Phys
Antonov AP, Terkel M, Schwarzendahl FJ, Rodríguez-Gallo C, Tierno P, Löwen H.   +5 more
europepmc   +1 more source

Free‐Volume Regulation Enables Significantly Enhanced Electrical Breakdown via Short‐Chain Molecular Spatial‐Positioning Intercalation into Poly(vinylidene fluoride)

Advanced Science, EarlyView.
An innovative molecular spatial‐positioning shimming strategy is developed to optimize the electrical properties of poly(vinylidene fluoride) (PVDF). By intercalating polyethylene wax (PE wax) short‐chain molecules into PVDF's free volume, the film achieves a significantly enhanced energy density of 32.67 J cm−3.
Ziyue Wang, Jiyang Xie, Wanbiao Hu
wiley   +1 more source

Narrative candour: Learning from diverse stories of imperfect medical practice. [PDF]

Med Educ
Bearman M, Molloy E, Varpio L.
europepmc   +1 more source

Origins of Graphite Resistivity: Decoupling Stacking Fault and Rotational Misorientation

Advanced Science, EarlyView.
Interfacial dislocations critically influence interlayer transport in van der Waals (vdW) materials, yet quantifying their individual contributions remains challenging. We measure graphite's c‐axis resistivity and develop a decoupling strategy, revealing a resistivity ratio of ∼4507:74:1 for rotational misorientations, stacking faults, and AB stacking,
Weipeng Chen, Fuwei Yang, Tielin Wu, Yelingyi Wang, Quanshui Zheng, Deli Peng, Zhanghui Wu   +6 more
wiley   +1 more source

Numerical study of imperfect liquid-filled conical shells - Verifying the present design rule [PDF]

, 2008
GUGGENBERGER, W, Lagae, Guy, Van Impe, Rudy, Vanlaere, Wesley   +3 more
core  

Shear and Compression Wrinkling Experimental Analysis with a Sandwich Beam Submitted to Three-Point Bending. [PDF]

Materials (Basel)
Gashe B, Alawode A, Rivallant S, Castanié B.   +3 more
europepmc   +1 more source