Results 51 to 60 of about 133,106 (256)

Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency

open access: yesBioTechniques, 1996
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
doaj   +1 more source

Molecular pathological detection of S. typhimurium by fluorescence in situ hybridization (FISH) in lambs tissue sections [PDF]

open access: yesJournal of Bioscience and Applied Research, 2018
Despite many different methods used to diagnose Salmonella typhimurium, especially that depended on isolation and biochemical identification, but use technique, Fluorescence in Situ Hybridization (FISH), as one method from molecular fashion probe, has ...
Basim Jwad, Bushra AL-Kaisei
doaj   +1 more source

Disentangling Bulk and Surface Contributions to Charge and Discharge Fading in High‐Voltage LiCoO2

open access: yesAdvanced Functional Materials, EarlyView.
High‐voltage operation of LiCoO2 accelerates capacity fading through bulk and surface degradation. By introducing a LiTaO3 coating to selectively suppress surface degradation, bulk and surface contributions to charge and discharge fading are decoupled.
Hyungjoon Moon   +6 more
wiley   +1 more source

Fluorescent in Situ Hybridization

open access: yesBangladesh Journal of Medical Biochemistry, 2018
Abstract not availableBangladesh J Med Biochem 2017; 10(1): 3 ...
S Akhter, Z Aziz, HB Asad
openaire   +2 more sources

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

open access: yesAdvanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma   +13 more
wiley   +1 more source

Longitudinal study on clinical and microbial analysis of periodontal status in pregnancy

open access: yesBrazilian Oral Research
This study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (FISH) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks ...
Fernanda Campos MACHADO   +4 more
doaj   +1 more source

Multicomponent Peptide‐MXene‐Hyaluronic Acid Hydrogel as a Conductive Scaffold for Enhanced Fibroblast Proliferation and Migration Under Electrical Stimulation

open access: yesAdvanced Healthcare Materials, EarlyView.
An electrically conductive FmocFF/MXene/hyaluronic acid hydrogel combines extracellular matrix‐like architecture, self‐healing behavior, oxidation‐resistant conductivity, and biochemical cues in a single scaffold. By enabling physiologically relevant electrical stimulation, the platform enhances fibroblast attachment, proliferation, and migration ...
Offir Loboda   +7 more
wiley   +1 more source

Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation

open access: yesMethodsX, 2019
DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large ...
Patrick J. McCoy   +4 more
doaj   +1 more source

Vascularization of Human iPSC‐Derived Kidney Organoids Using Perfusion Culture, Pre‐Vascularized Collagen Scaffolds, and Decellularized Extracellular Matrix

open access: yesAdvanced Healthcare Materials, EarlyView.
An integrated perfusion‐based culture platform combining pre‐vascularized collagen scaffolds, stromal vascular fraction cells, and kidney decellularized extracellular matrix promotes vascularization of human iPSC‐derived kidney organoids. The platform provides evidence of interactions between vascular networks and glomerular‐like structures and enables
Helen Kearney   +5 more
wiley   +1 more source

Nanomaterial Strategies for Pulmonary Delivery of Immunotherapeutics in Lung Cancer Treatment

open access: yesAdvanced Healthcare Materials, EarlyView.
Inhalable immunotherapeutic nanomedicines enable organ‐selective immune modulation by overcoming pulmonary delivery barriers and concentrating therapy within lung tumors. This Review defines how nanomaterial properties govern airway deposition, retention, cellular partitioning, and immune activation across vaccines, checkpoint blockade, STING agonists,
Han Zhang, Wei Tang
wiley   +1 more source

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