Results 221 to 230 of about 113,971 (265)
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1971
The first attempts using tissue culture techniques to study in vitro multiplications of the aster yellows agent in its leafhopper tissues were carried out by Maramorosch (1956). Since neither adequate culture medium nor specific techniques were available at that time, he simply used body fragments of yellows-infected nymphs of M. fascifrons.
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The first attempts using tissue culture techniques to study in vitro multiplications of the aster yellows agent in its leafhopper tissues were carried out by Maramorosch (1956). Since neither adequate culture medium nor specific techniques were available at that time, he simply used body fragments of yellows-infected nymphs of M. fascifrons.
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Continuous in vitro cultivation of Babesia gibsoni
Parasitology Research, 2000Two stocks of the protozoan parasite Babesia gibsoni, one of the causative agents of canine piroplasmosis, were propagated continuously in dog erythrocytes in microaerophilous stationary-phase culture. Cultures of both stocks were initiated in a humidified 5% CO2, 2% O2, 93% N2 atmosphere at 37 degrees C at a time when very few parasites (
E, Zweygarth, L M, Lopez-Rebollar
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In vitro Cultivation of Human Tumor Tissues
Oncology, 2009In order to develop cell substrates suitable for the isolation of human oncornaviruses, a large number of human tumors, mostly sarcomas, were cultured in vitro. All explants yielded primary outgrowth of cells. These were of various nature and morphology and exhibited different growth potentials.
A, Billiau +4 more
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Continuous In vitro Cultivation of Sarcocystis cruzi
The Journal of Parasitology, 1990Sporozoites of Sarcocystis cruzi were inoculated onto monolayer cultures of bovine pulmonary artery endothelial (CPA) cells. Sporozoites entered the cells, formed large and small multinucleate schizonts, and produced large numbers of merozoites. Continuous cultivation from the original sporozoite inoculum has been maintained for more than 1,320 days by
C D, Andrews, R, Fayer, J P, Dubey
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Cultivation of Adult Human Iris in Vitro
Archives of Ophthalmology, 1958The clinical observation that the iris does not show any evidence of regeneration of its tissue elements following aseptic iridectomy has never been satisfactorily explained. Up to date there have been very few studies on the healing process of the human iris and only little experimental work on this subject in animals.
E, TENENBAUM, W, KORNBLUETH
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In vitro cultivation of Eimeria acervulina (Coccidia)
Experimental Parasitology, 1965Abstract Eimeria acervulina oocysts were sterilized with 50 ppm chlorine and viable sporozoites were released mechanically with the aid of trypsin and bile. These sporozoites, when suspended in the appropriate cell culture nutrient, were used as inoculum for chick embryo kidney, chick embryo fibroblast, mouse fibroblast, human amnion, and HeLa cell
R G, Strout +3 more
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The Cultivation of Cells in Vitro
1984Tissue culture techniques are widely used in the study of cell locomotion, since they make it possible to observe living cells and record their behaviour. It was the need to make the growth of embryonic nerve fibres visible which led Ross G. Harrison to devise, in 1907, the first successful method for the cultivation of living tissue outside the body ...
C. A. Middleton, J. A. Sharp
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Human Wart Virus: In vitro Cultivation
Science, 1964Inoculation of cell cultures of fetal skin of human and murine origin with virus extracted from human wart tissue resulted in the appearance of intracellular wart virus specific antigen, as demonstrated by fluorescent antibody techniques. Appearance of antigen was accompanied by cytopathogenicity and the accumulation of large numbers of characteristic ...
S, OROSZLAN, M A, RICH
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Continuous In Vitro Cultivation of Babesia bovis
American Journal of Veterinary Research, 1980SUMMARY Babesia bovis was isolated from an experimentally infected calf (No. 1) and was maintained in vitro for 32 days by subculturing 14 times, using a total dilution of 192,000. A splenectomized calf was inoculated with subculture Babesia (isolate B).
E E, Erp +3 more
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Cultivation in vitro of Plasmodium gallinaceum oocysts
Experimental Parasitology, 1968Abstract Large numbers of viable infectious Plasmodium gallinaceum sporozoites, virtually free of mosquito tissue, were obtained by culturing somewhat younger stages of the sporogonous cycle. Twenty or more oocysts were placed in each hanging- or sitting-drop culture.
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