Results 21 to 30 of about 113,971 (265)

In vitro Cultivation of Porcine Kidney

Experimental Biology and Medicine, 1956
Summary and Conclusions1. Some of the nutritional requirements for the in vitro cultivation of hog kidney epithelial cells were determined. The data indicate that cultures grown in a “simple” medium containing Synthetic Mixture #199 (92.5%) and hog serum (7.5%) were consistently more proliferative than cultures cultivated in more complex media ...
S J, MILLIAN, K C, ROBBINS
openaire   +2 more sources

THE CULTIVATION OF THE PLASMODIUM FALCIPARUM IN VITRO [PDF]

Journal of the American Medical Association, 1914
In 1911, C. C. Bass 1 of New Orleans reported the successful cultivation of the plasmodium of malaria in vitro . He stated that he had been able to accomplish this by maintaining anaerobic conditions and a temperature of 40 C. (104 F.). Following these suggestions, Sinton 2 of London as reported in August, 1912, attempted the growth of the organisms ...
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

Ciência Rural, 2018
: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks.
Natália Helena Gavilan, Fernanda Cardoso Furlan, Alex Zichner Zorz, Leandro Silva de Oliveira, Wellington Ferreira Campos, Gilvano Ebling Brondani   +5 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

THE CULTIVATION OF LENS EPITHELIUM IN VITRO [PDF]

Journal of Experimental Medicine, 1927
1. Differentiation of the posterior cells of the lens vesicle into lens cortex has been observed in vitro. 2. It is possible to dissect out the lens of the 5 day embryo chick in such a manner as to free it from all extraneous cells. 3. Lens epithelium even at a very early stage is contained within a cuticula.
openaire   +2 more sources

Adaptor protein CIN85 potentiates the motility of osteosarcoma cells via the Akt/mTOR and MMP2‐COL3A1 axis

Molecular Oncology, EarlyView.
CIN85 is highly expressed in osteosarcoma, particularly in metastatic lesions. Its overexpression increases cell migration and Matrigel invasion, while silencing CIN85 suppresses these behaviors. Transcriptome analysis shows that CIN85 regulates MMP2, COL3A1, and Akt/mTOR signaling. Targeting these pathways reverses CIN85‐induced motility, highlighting
Iryna Horak, Iva Staniczková Zambo, Matěj Přikryl, Peter Múdry, Danica Zapletalová, Jarmila Navrátilová, Jiří Navrátil, Jan Šmarda, Liudmyla Drobot, Petr Beneš, Lucia Knopfová   +10 more
wiley   +1 more source

Improved and easy method for long-term in vitro culture of rumen ciliates Dasytricha ruminantium

MethodsX
The rumen ciliates are a diverse group of protozoa residing in the rumen of ruminant animals. They are primarily found in the orders Entodiniomorphida and Vestibuliferida, playing crucial roles in the digestion and breakdown of feed within the host's ...
Qinhui Xu, Jinying He, Yujia Wang, Jie Xiong, Weiwei Qin, Jinmei Feng   +5 more
doaj   +1 more source

Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions

Вавиловский журнал генетики и селекции, 2019
Cryopreservation provides long-term storage of the gene pool of potato varieties in cryobanks at extremely low temperatures. Currently, droplet vitrification is the most widely used method for cryopreservation of potato varieties, which is constantly ...
E. S. Bespalova, Yu. V. Ukhatova, N. N. Volkova, E. V. Oves, N. A. Gaitova, T. A. Gavrilenko   +5 more
doaj   +1 more source