Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]
The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Aldave, Anthony J +14 more
core
A 3-D in vitro co-culture model of mammary gland involution. [PDF]
Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring.
Botos, Laur-Alexandru +5 more
core +3 more sources
We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval.
Takumi YOSHIDA +8 more
doaj +1 more source
An Extended Culture System that Supports Human Primordial Germ Cell-like Cell Survival and Initiation of DNA Methylation Erasure. [PDF]
The development of an in vitro system in which human primordial germ cell-like cells (hPGCLCs) are generated from human pluripotent stem cells (hPSCs) has been invaluable to further our understanding of human primordial germ cell (hPGC) specification ...
Bower, Grace +9 more
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Echinococcus, Giardia and Cryptosporidium: observational studies challenging accepted dogma [PDF]
The development of in vitro culture systems that allow the maintenance, and support the development of Echinococcus, Giardia and Cryptosporidium in the laboratory have had a significant impact on their biology and taxonomy and the epidemiology of ...
Thompson, R.C.A.
core +2 more sources
GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays
The temporary immersion system bioreactor named GreenTray® presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, nor is the use of a scalpel necessary to divide ...
Ramon Dolcet-Sanjuan +8 more
doaj +1 more source
In Vitro Porcine Colon Culture
Models have been extensively used to investigate disease pathogenesis. Animal models are costly, require extensive logistics for animal care, and samples are not always suitable for different analytical techniques or to answer the research question. In vitro cell culture models are generally focused on recreating a specific characteristic of an organ ...
Costa, Matheus O. +3 more
openaire +4 more sources
Anabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages [PDF]
Oxygen is a critical parameter proposed to modulate the functions of chondrocytes ex-vivo as well as in damaged joints. This article investigates the effect of low (more physiological) oxygen percentage on the biosynthetic and catabolic activity of human
Ströbel, Simon +8 more
core +2 more sources
Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus spp. Embryos
The major factors affecting the in vitro immature embryo rescue efficiencies from Prunus persica or P. armeniaca accessions have been identified, along with improving the feasibility.
Maria Casanovas +2 more
doaj +1 more source
Culture medium geometry. The dominant factor affecting in vitro RF exposure dosimetry [PDF]
Biological experiments that expose living cells or tissues to RF energy must have an aqueous medium to provide essential water, ions, nutrients, and growth factors.
Apollonio, Francesca +5 more
core +3 more sources

