Results 41 to 50 of about 115,167,710 (246)

A Validated Multiplex Real-Time PCR Assay for the Diagnosis of Infectious Leptospira spp.: A Novel Assay for the Detection and Differentiation of Strains From Both Pathogenic Groups I and II

Frontiers in Microbiology, 2020
Leptospirosis is recognized as the most globally widespread reemerging zoonosis and represents a serious threat for both human and animal health. Indeed, leptospirosis is linked to more than 60,000 human deaths per year and to incalculable economic ...
Lester J. Pérez, Lester J. Pérez, Saraswathi Lanka, Vanessa J. DeShambo, Richard L. Fredrickson, Carol W. Maddox   +5 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Post-market surveillance for literature on QuantiFERON TB Gold Plus: what improvements can artificial intelligence bring?

IJTLD Open
BACKGROUND: Post-market surveillance (PMS) under the European Union In Vitro Diagnostic Regulation (IVDR) demands proactive, literature-based evidence, but mature assays like QuantiFERON TB Gold Plus (QFT-Plus) generate volumes of peer-reviewed and other
J. Reniewicz, R. Alagna, L. Kordylas, M. Latacz, V. Suryaprakash, U. Nowak, A. Kois-Ostrowska, J. Weleszczuk, A. Blacha, V. Nikolayevskyy   +9 more
doaj   +1 more source

Detection and Quantification of the Oomycete Saprolegnia parasitica in Aquaculture Environments

Microorganisms, 2022
Saprolegnia parasitica induces heavy mortality in aquaculture. The detection of S. parasitica is often time consuming and uncertain, making it difficult to manage the disease. We validated a previously published real-time quantitative PCR (qPCR) assay to
Tiina Korkea-aho, Tom Wiklund, Christine Engblom, Anssi Vainikka, Satu Viljamaa-Dirks   +4 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea [PDF]

, 2010
Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for ...
Barnadas, Céline, Siba Peter, Betuela Inoni, Mueller Ivo, Zimmerman, Peter A., Rosanas-Urgell, Anna, Felger Ingrid, Felger, Ingrid, Rosanas-Urgell Anna, Dania Mueller, Mueller, Dania, Céline Barnadas, Ingrid Felger, Del Portillo, HA, Iga, Jonah, Felger, I, Hernando A del Portillo, Mueller, Ivo, Mueller Dania, Mueller, D, Anna Rosanas-Urgell, Siba, P, del Portillo, Hernando A., del Portillo Hernando A, Ivo Mueller, Iga Jonah, Peter Siba, Zimmerman, PA, Siba, Peter, Barnadas, C, Betuela, Inoni, Mueller, I, Barnadas Céline, Inoni Betuela, Jonah Iga, Betuela, I, Zimmerman Peter A, Peter A Zimmerman, Iga, J, Rosanas-Urgell, A   +39 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes

BMC Bioinformatics, 2007
Background DNA melting curve analysis using double-stranded DNA-specific dyes such as SYTO9 produce complex and reproducible melting profiles, resulting in the detection of multiple melting peaks from a single amplicon and allowing the discrimination of ...
Saint Christopher P, Rasmussen John P, Monis Paul T   +2 more
doaj   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source