Viscous sputum specimens usually cannot undergo automated extraction, and thus, a pre-homogenization process is desirable before isolating nucleic acids for real-time reverse transcription PCR.
Fei Yu +6 more
doaj +1 more source
Intramachine and intermachine variability in transesophageal color doppler images of pulsatile jets: in vitro studies [PDF]
Background: Color Doppler flow mapping is widely used as a marker of severity of valvular regurgitation, and the transesophageal approach has provided high-quality images in patients with poor acoustic windows.
Anayiotos, Andreas +2 more
core +2 more sources
miRNA-based rapid differentiation of purified neurons from hPSCs advancestowards quick screening for neuronal disease phenotypes in vitro [PDF]
Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs).
Aoyama, Takeshi +10 more
core +2 more sources
Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis [PDF]
The homing of Endothelial Progenitor Cells (EPCs) to tumor angiogenic sites has been described as a multistep process, involving adhesion, migration, incorporation and sprouting, for which the underlying molecular and cellular mechanisms are yet to be ...
Bieback, Karen +6 more
core +1 more source
Molecular medicine of microRNAs: structure, function and implications for diabetes [PDF]
MicroRNAs (miRNAs) are a family of endogenous small noncoding RNA molecules, of 19–28 nucleotides in length. In humans, up to 3% of all genes are estimated to encode these evolutionarily conserved sequences.
Duncan +5 more
core +1 more source
In vitro Models of the Small Intestine for Studying Intestinal Diseases
The small intestine is a digestive organ that has a complex and dynamic ecosystem, which is vulnerable to the risk of pathogen infections and disorders or imbalances.
Sang-Myung Jung, Seonghun Kim
semanticscholar +1 more source
Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells [PDF]
To obtain a useful rat liver cell line for in vitro carcinogenesis, two rat diploid epithelial cell lines were established from a 7-day-old male rat by the repeated colonial clone method.
Masuji, Hiroshi +2 more
core +1 more source
TEER Measurement Techniques for In Vitro Barrier Model Systems
Transepithelial/transendothelial electrical resistance (TEER) is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers.
B. Srinivasan +5 more
semanticscholar +1 more source
In-silico Predictive Mutagenicity Model Generation Using Supervised Learning Approaches [PDF]
With the advent of High Throughput Screening techniques, it is feasible to filter possible leads from a mammoth chemical space that can act against a particular target and inhibit its action.
Abhik Seal +4 more
core +2 more sources
Production of Pigs From Porcine Embryos Generated in vitro
Generating porcine embryos in vitro is a critical process for creating genetically modified pigs as agricultural and biomedical models; however, these embryo technologies have been scarcely applied by the swine industry. Currently, the primary issue with
P. Chen +5 more
semanticscholar +1 more source

