Results 51 to 60 of about 690,329 (266)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Physicomechanical characterization and biological evaluation of bulk-fill composite resin

Brazilian Oral Research, 2018
The aim of this study was to evaluate the cytotoxic effect, degree of conversion (% DC), Vickers hardness (VH), and surface morphology of composite resins. Eleven resins, nine bulk-fill resins, and two conventional resins were evaluated.
Armiliana Soares NASCIMENTO, Daniel Bezerra LIMA, Marcus Vinicius Lia FOOK, Monica Soares de ALBUQUERQUE, Eliane Alves de LIMA, Marcos Antonio SABINO, Silvia Maria Pinto BORGES, Pedro Tardelly Diniz FILGUEIRA, Yasmine Carvalho de SOUSA, Rodivan BRAZ   +9 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System [PDF]

International Journal of Fertility and Sterility, 2010
Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality ...
Mohsen Forouzanfar, Sayyed Mortaza Hosseini, Mehdi Hajian, Fariba Molavi, Parvaneh Abedi, Mohammad Hossein Nasr Esfahani   +5 more
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

In Vitro Breeding Techniques for Alstroemeria [PDF]

HortScience, 1996
Alstroemeria , also known as Lily-of-the-Incas, Inca Lily, or Peruvian Lily, has been bred at the Univ. of Connecticut since 1985. In vitro procedures have been integrated with traditional breeding techniques to create new and exciting cultivars.
openaire   +1 more source

In vitro rumen feed degradability assessed with DaisyII and batch culture: effect of sample size

Italian Journal of Animal Science, 2010
In vitro degradability with DaisyII (D) equipment is commonly performed with 0.5g of feed sample into each filter bag. Literature reported that a reduction of the ratio of sample size to bag surface could facilitate the release of soluble or fine ...
Stefano Schiavon, Lucia Bailoni, Franco Tagliapietra, Mirko Cattani   +3 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Metabolomic profiling of plasma reveals differential disease severity markers in avian influenza A(H7N9) infection patients

International Journal of Infectious Diseases
Objectives: Avian influenza such as H7N9 is currently a major global public health risk, and at present, there is a lack of relevant diagnostic and treatment markers. Methods: We collected plasma samples from 104 confirmed H7N9 patients, 31 of whom died.
Yuefeng Wang, Jili Ni, Mingzhu Huang, Wenxin Qu, Chang Liu, Zheying Mao, Jiaqi Bao, Weizhen Chen, Dongsheng Han, Fei Yu, Yifei Shen, Zhenzhen Deng, Shufa Zheng   +12 more
doaj   +1 more source