Frontiers in Microbiology, 2017 Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen.
Haishen Kong +5 more
doaj +1 more source
miRNA-based rapid differentiation of purified neurons from hPSCs advancestowards quick screening for neuronal disease phenotypes in vitro [PDF]
, 2020 Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs).
Aoyama, Takeshi +10 more
core +2 more sources
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy [PDF]
, 2014 Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms.
Adam T. Watson +46 more
core +1 more source
Influenza A-associated severe pneumonia in hospitalized patients: Risk factors and NAI treatments
International Journal of Infectious Diseases, 2020 Objective: The risk factors and the impact of NAI treatments in patients with severe influenza A-associated pneumonia remain unclear. Methods: A multicenter, retrospective, observational study was conducted in Zhejiang, China during a severe influenza ...
Qianda Zou +20 more
doaj +1 more source
An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]
, 2015 The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael +2 more
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An in vivo/vitro embryo culture technique [PDF]
Hereditas, 2009 Work has been carried out for a number of years on the development of a new and improved embryo culture technique to supplement or replace the traditional in vitro methods. This new method is based on the implantation of hybrid embryos onto naked Hordeum endosperm placed on a normal growth medium.
openaire +2 more sources
124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics [PDF]
, 2019 Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive.
Ganji, M. +13 more
core +3 more sources
TECHNIQUE OF CULTIVATING HUMAN TISSUES IN VITRO [PDF]
Journal of Experimental Medicine, 1916 1. Unmodified human plasma is not a satisfactory culture medium for human tissues owing to the susceptibility of human fibrin to digestion by tissue ferments. The necessary framework is thus destroyed before the cells begin to migrate. The difficulty can be overcome by adding to human plasma or serum a small quantity of fowl or pigeon plasma, the ...
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Biomimetic bone-like apatite coating on anodised titanium in simulated body fluid under UV irradiation [PDF]
, 2017 Low temperature deposition techniques of bioceramics coatings are now being researched and developed to avoid deficiencies inherent in high temperature techniques.
Chandrasekar Ramasamy, Chandrasekar Ramasamy +4 more
core +1 more source
Techniques to Study Transendothelial Migration In Vitro
Klinicka onkologie, 2014 The most dangerous aspect of cancer is the metastatic spread to other parts of the body. Cancer cells frequently use circulation to spread to secondary locations. By entering the blood-stream (in a process called intravasation) and by crossing the vessel walls at the metastatic sites (extravasation) tumor cells disseminate to distal organs and ...
L, Knopfová, P, Bouchal, J, Smarda
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