Results 1 to 10 of about 549 (211)
Radiolabeling of functional oligonucleotides for molecular imaging
Frontiers in Bioengineering and Biotechnology, 2022 Molecular imaging has greatly advanced basic biology and translational medicine through visualization and quantification of molecular events in a cellular context and living organisms.
Dunfang Liu +7 more
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Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides [PDF]
Molecules, 2020 In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications.
Steven Ochoa, Valeria T. Milam
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Molecules, 2023 Enzymatic oligonucleotide synthesis is used for the development of functional oligonucleotides selected by in vitro selection. Expanding available sugar modifications for in vitro selection helps the functional oligonucleotides to be used as therapeutics
Kenta Ishida +4 more
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Assessment of enzymatically synthesized DNA for gene assembly
Frontiers in Bioengineering and Biotechnology, 2023 Phosphoramidite chemical DNA synthesis technology is utilized for creating de novo ssDNA building blocks and is widely used by commercial vendors. Recent advances in enzymatic DNA synthesis (EDS), including engineered enzymes and reversibly terminated ...
Brooke L. Simmons +4 more
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Molecular Therapy: Nucleic Acids, 2021 Intracellular delivery of oligonucleotides is important for their use as therapeutic drugs. The conjugation of molecules interacting with cell membrane proteins to enhance their internalization into cells is an effective strategy for delivering ...
Keisuke Tanaka +3 more
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Inhibition of microRNA function by antimiR oligonucleotides [PDF]
Silence, 2012 Abstract MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in many developmental and cellular processes. Moreover, there is now ample evidence that perturbations in the levels of individual or entire families of miRNAs are strongly associated with the pathogenesis of a wide range of human diseases.
Stenvang, Jan +4 more
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Stereospecificity of oligonucleotide interactions revisited: no evidence for heterochiral hybridization and ribozyme/DNAzyme activity. [PDF]
PLoS ONE, 2015 A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and molecular medicine is their susceptibility to abundant nucleases. One intriguing possibility to tackle this problem is the use of mirror-image (l-)oligonucleotides.
Kai Hoehlig +2 more
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Pharmaceutics, 2023 Oligonucleotide conjugates are versatile scaffolds that can be applied in DNA-based screening platforms and ligand display or as therapeutics. Several different chemical approaches are available for functionalizing oligonucleotides, which are often ...
Zoltán Kupihár +6 more
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Asymmetric Functionalization of Gold Nanoparticles with Oligonucleotides [PDF]
Journal of the American Chemical Society, 2006 Gold nanoparticles (AuNPs) were anisotropically functionalized with two different oligonucleotide sequences using magnetic microparticles as geometric restriction templates for site-selective enzymatic extension of particle-bound oligonucleotides. The divalent linking capability of the resulting AuNPs allowed for the design and programmable assembly of
Xiaoyang, Xu +4 more
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Functionalization of Surfaces with Synthetic Oligonucleotides [PDF]
, 2011 There is a large interest in the use of nucleic acids covalently bound to surfaces for a variety of biomedical uses: biosensors, microarrays, drug delivery, lab-on-chip devices, and gene therapy, etc. Most of these applications require the covalent attachment of oligonucleotides via specific reactive groups on both modified oligonucleotide and/or ...
Manning, Brendan D. +1 more
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