Radiolabeling of functional oligonucleotides for molecular imaging [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Molecular imaging has greatly advanced basic biology and translational medicine through visualization and quantification of molecular events in a cellular context and living organisms.
Dunfang Liu +7 more
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Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides [PDF]
Molecules, 2020 In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications.
Steven Ochoa, Valeria T. Milam
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Yeast Efficiently Utilizes Ribosomal RNA-Derived Oligonucleotides as Bioavailable Nutrient Sources [PDF]
FoodsNucleic acids are essential dietary components with diverse physiological functions. Numerous studies have focused on the biological functions of nucleotides, nucleosides, and functional RNAs such as microRNAs. However, the nutritional value of ribosomal
Xinmei Du +7 more
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Position-Dependent Stabilization of DNA/RNA Duplexes by Site-Specific Incorporation of LNA Nucleosides [PDF]
Journal of Nucleic AcidsOwing to their enhanced functional properties, 2′,4′-bridged nucleic acids/locked nucleic acids (2′,4′-BNAs/LNAs) are considered promising candidates for antisense oligonucleotide therapeutics.
Elisa Tomita-Sudo +6 more
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Identification and functional validation of intracellular protein partners of phosphorothioate splice-switching oligonucleotides using AP-MS [PDF]
Molecular Therapy: Nucleic AcidsCellular uptake and intracellular distribution of phosphorothioate-modified antisense oligonucleotides (PS-ASOs) are mediated by protein interactions.
Aris Gaci +5 more
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Molecules, 2023 Enzymatic oligonucleotide synthesis is used for the development of functional oligonucleotides selected by in vitro selection. Expanding available sugar modifications for in vitro selection helps the functional oligonucleotides to be used as therapeutics
Kenta Ishida +4 more
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Assessment of enzymatically synthesized DNA for gene assembly
Frontiers in Bioengineering and Biotechnology, 2023 Phosphoramidite chemical DNA synthesis technology is utilized for creating de novo ssDNA building blocks and is widely used by commercial vendors. Recent advances in enzymatic DNA synthesis (EDS), including engineered enzymes and reversibly terminated ...
Brooke L. Simmons +4 more
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Electropolymerizable Thiophene–Oligonucleotides for Electrode Functionalization
ACS Applied Materials & Interfaces, 2022 Inserting complex biomolecules such as oligonucleotides during the synthesis of polymers remains an important challenge in the development of functionalized materials. In order to engineer such a biofunctionalized interface, a single-step method for the covalent immobilization of oligonucleotides (ONs) based on novel electropolymerizable lipid ...
Kassahun, Getnet +9 more
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Molecular Therapy: Nucleic Acids, 2021 Intracellular delivery of oligonucleotides is important for their use as therapeutic drugs. The conjugation of molecules interacting with cell membrane proteins to enhance their internalization into cells is an effective strategy for delivering ...
Keisuke Tanaka +3 more
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Stereospecificity of oligonucleotide interactions revisited: no evidence for heterochiral hybridization and ribozyme/DNAzyme activity. [PDF]
PLoS ONE, 2015 A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and molecular medicine is their susceptibility to abundant nucleases. One intriguing possibility to tackle this problem is the use of mirror-image (l-)oligonucleotides.
Kai Hoehlig +2 more
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