Results 11 to 20 of about 549 (211)
Gold nanocarriers for transport of oligonucleotides across brain endothelial cells.
PLoS ONE, 2020 Treatment of diseases that affect the CNS by gene therapy requires delivery of oligonucleotides to target cells within the brain. As the blood brain barrier prevents movement of large biomolecules, current approaches involve direct injection of the ...
Nayab Fatima +4 more
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Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
Pharmaceutics, 2020 MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA ...
Asako Yamayoshi +9 more
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Chemistry, structure and function of approved oligonucleotide therapeutics
Nucleic Acids Research, 2023 Abstract Eighteen nucleic acid therapeutics have been approved for treatment of various diseases in the last 25 years. Their modes of action include antisense oligonucleotides (ASOs), splice-switching oligonucleotides (SSOs), RNA interference (RNAi) and an RNA aptamer against a protein. Among the diseases targeted by this new class of
Martin Egli, Muthiah Manoharan
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Synthesis and evaluation of the functional oligonucleotides-PEG conjugates [PDF]
Nucleic Acids Symposium Series, 2004 We have previously demonstrated that the phenylsulfide and phenylsulfoxide derivatives of 2-amino-6-vinylpurine exhibit efficient cross-linking with high selectivity towards cytidine through synchronous activation within duplex. In this study, we synthesized their conjugates with PEG for possible application to in vitro as well as in vivo antisense ...
Ali Md, Monsur +5 more
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PNA-Dependent Gene Chemistry: Stable Coupling of Peptides and Oligonucleotides to Plasmid DNA
BioTechniques, 2000 Two approaches are described for stably conjugating peptides, proteins and oligonucleotides onto plasmid DNA. Both methods use a peptide nucleic acid (PNA) clamp, which binds irreversibly and specifically to a binding site cloned into the plasmid.
O. Zelphati +6 more
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Life, 2022 The role of minerals in the chemical evolution of RNA molecules is an important issue when considering the early stage of the Hadean Earth. In particular, the interaction between functional ribozymes and ancient minerals under simulated primitive ...
Kunio Kawamura +5 more
doaj +1 more source
Nature Communications, 2020 Restoration of normal gene expression is one way to treat monogenic disorders. Here the authors target naturally occurring non-productive alternative splicing using antisense oligonucleotides to promote the production of functional proteins.
Kian Huat Lim +15 more
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Automated in vitro selection to obtain functional oligonucleotides [PDF]
Nucleic Acids Symposium Series, 2000 In vitro selection or systematic evolution of ligand by exponential enrichment (SELEX) has been devised for the identification of high-affinity oligonucleotide aptamers to target molecules. However, the selection process is repetitive and time-consuming.
H, Zhang +4 more
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Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain
Molecular Therapy: Nucleic Acids, 2015 Applications of RNA interference for neuroscience research have been limited by a lack of simple and efficient methods to deliver oligonucleotides to primary neurons in culture and to the brain.
Julia F Alterman +13 more
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Molecules, 2017 In this review, we summarize the recent advances in the use of pyrene-modified oligonucleotides as a platform for functional nucleic acid-based constructs.
Olga A. Krasheninina +3 more
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