Results 21 to 30 of about 549 (211)

The Functionalization of Oligonucleotides Via Phosphoramidite Derivatives [PDF]

Tetrahedron, 1993
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option.
Beaucage, Serge L., Iyer, Radhakrishnan P.   +1 more
openaire   +1 more source

A Simple and Efficient Microfluidic System for Reverse Chemical Synthesis (5′-3′) of a Short-Chain Oligonucleotide Without Inert Atmosphere

Applied Sciences, 2019
Reverse DNA synthesis (5′-3′) plays diverse functional roles in cellular biology, biotechnology, and nanotechnology. However, current microfluidic systems for synthesizing single-stranded DNAs at a laboratory scale are limited.
Rahul Bhardwaj, Phan T. Tue, Manish Biyani, Yuzuru Takamura   +3 more
doaj   +1 more source

Yeast Efficiently Utilizes Ribosomal RNA-Derived Oligonucleotides as Bioavailable Nutrient Sources

Foods
Nucleic acids are essential dietary components with diverse physiological functions. Numerous studies have focused on the biological functions of nucleotides, nucleosides, and functional RNAs such as microRNAs. However, the nutritional value of ribosomal
Xinmei Du, Qitao Chen, Jingyun Zhuang, Mingqi Zhao, Yixin Duan, Shuang Wang, Ran An, Xingguo Liang   +7 more
doaj   +1 more source

Truncated Amplification: A Method for High-Fidelity Template-Driven Nucleic Acid Amplification

BioTechniques, 2002
The error rate of conventional PCR is problematic when amplifying from single cells or amplifying segments for protein functional analysis by in vitro translation. We describe truncated amplification, a method for high-fidelity amplification in which DNA
Qiang Liu, Piotr Swiderski, Steve S. Sommer   +2 more
doaj   +1 more source

Encoding function into polypeptide-oligonucleotide precision biopolymers

Chemical Communications, 2018
We report a novel synthesis strategy to prepare precision polymers providing exact chain lengths, molecular weights and monomer sequences that allow post modifications by convenient DNA hybridization.
Weina Liu, Felix Boldt, Yu Tokura, Tao Wang, Bikram Keshari Agrawalla, Yuzhou Wu, Tanja Weil   +6 more
openaire   +4 more sources

Photo-regulation of DNA function by azobenzene-tethered oligonucleotides [PDF]

Nucleic Acids Symposium Series, 2003
Multiple azobenzene moieties were tethered to DNA on D-threoninol linker, and formation and dissociation of DNA duplex could be reversibly photo-regulated either by irradiating UV or visible light. With this photo-responsive DNA, RNase H reaction was also successfully photo-regulated.
Hiroyuki, Asanuma, Daijiro, Matsunaga, Minghe, Liu, Xingguo, Liang, Jing, Jhao, Makoto, Komiyama   +5 more
openaire   +2 more sources

Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer

Molecular Oncology, EarlyView.
Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni, Antonella Palmese, Stefano Scalera, Laura Cipriani, Davide Mascolo, Patrizia Vici, Teresa Arcuri, Lorena Filomeno, Eriseld Krasniqi, Giovanni Blandino, Giulia Bon, Marcello Maugeri‐Saccà   +11 more
wiley   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Modified Nucleoside Triphosphates for in-vitro Selection Techniques

Frontiers in Chemistry, 2016
The development of SELEX (Selective Enhancement of Ligands by Exponential Enrichment) provides a powerful tool for the search of functional oligonucleotides with the ability to bind ligands with high affinity and selectivity (aptamers) and for the ...
Adolfo Marcelo Iribarren, Adolfo Marcelo Iribarren, María eDellafiore, Javier Marcelo Montserrat, Javier Marcelo Montserrat   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source