Results 21 to 30 of about 179,623 (257)

Improved Metal-Free Approach for the Synthesis of Protected Thiol Containing Thymidine Nucleoside Phosphoramidite and Its Application for the Synthesis of Ligatable Oligonucleotide Conjugates

Pharmaceutics, 2023
Oligonucleotide conjugates are versatile scaffolds that can be applied in DNA-based screening platforms and ligand display or as therapeutics. Several different chemical approaches are available for functionalizing oligonucleotides, which are often ...
Zoltán Kupihár, Györgyi Ferenc, Vencel L. Petrovicz, Viktória R. Fáy, Lajos Kovács, Tamás A. Martinek, Zsófia Hegedüs   +6 more
doaj   +1 more source

Inhibition of microRNA function by antimiR oligonucleotides [PDF]

Silence, 2012
Abstract MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in many developmental and cellular processes. Moreover, there is now ample evidence that perturbations in the levels of individual or entire families of miRNAs are strongly associated with the pathogenesis of a wide range of human diseases.
Stenvang, Jan, Petri, Andreas, Lindow, Morten, Obad, Susanna, Kauppinen, Sakari   +4 more
openaire   +4 more sources

Gold nanocarriers for transport of oligonucleotides across brain endothelial cells.

PLoS ONE, 2020
Treatment of diseases that affect the CNS by gene therapy requires delivery of oligonucleotides to target cells within the brain. As the blood brain barrier prevents movement of large biomolecules, current approaches involve direct injection of the ...
Nayab Fatima, Radka Gromnicova, Jane Loughlin, Basil Sharrack, David Male   +4 more
doaj   +2 more sources

Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA

Pharmaceutics, 2020
MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA ...
Asako Yamayoshi, Shota Oyama, Yusuke Kishimoto, Ryo Konishi, Tsuyoshi Yamamoto, Akio Kobori, Hiroshi Harada, Eishi Ashihara, Hiroshi Sugiyama, Akira Murakami   +9 more
doaj   +1 more source

Functionalization of Surfaces with Synthetic Oligonucleotides [PDF]

, 2011
There is a large interest in the use of nucleic acids covalently bound to surfaces for a variety of biomedical uses: biosensors, microarrays, drug delivery, lab-on-chip devices, and gene therapy, etc. Most of these applications require the covalent attachment of oligonucleotides via specific reactive groups on both modified oligonucleotide and/or ...
Manning, Brendan, Eritja Casadellà, Ramón   +1 more
openaire   +3 more sources

Asymmetric Functionalization of Gold Nanoparticles with Oligonucleotides [PDF]

Journal of the American Chemical Society, 2006
Gold nanoparticles (AuNPs) were anisotropically functionalized with two different oligonucleotide sequences using magnetic microparticles as geometric restriction templates for site-selective enzymatic extension of particle-bound oligonucleotides. The divalent linking capability of the resulting AuNPs allowed for the design and programmable assembly of
Xiaoyang, Xu, Nathaniel L, Rosi, Yuhuang, Wang, Fengwei, Huo, Chad A, Mirkin   +4 more
openaire   +2 more sources

PNA-Dependent Gene Chemistry: Stable Coupling of Peptides and Oligonucleotides to Plasmid DNA

BioTechniques, 2000
Two approaches are described for stably conjugating peptides, proteins and oligonucleotides onto plasmid DNA. Both methods use a peptide nucleic acid (PNA) clamp, which binds irreversibly and specifically to a binding site cloned into the plasmid.
O. Zelphati, X. Liang, C. Nguyen, S. Barlow, S. Sheng, Z. Shao, P.L. Felgner   +6 more
doaj   +1 more source

Life on Minerals: Binding Behaviors of Oligonucleotides on Zirconium Silicate and Its Inhibitory Activity for the Self-Cleavage of Hammerhead Ribozyme

Life, 2022
The role of minerals in the chemical evolution of RNA molecules is an important issue when considering the early stage of the Hadean Earth. In particular, the interaction between functional ribozymes and ancient minerals under simulated primitive ...
Kunio Kawamura, Jean-François Lambert, Louis M. P. Ter-Ovanessian, Jacques Vergne, Guy Hervé, Marie-Christine Maurel   +5 more
doaj   +1 more source

Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression

Nature Communications, 2020
Restoration of normal gene expression is one way to treat monogenic disorders. Here the authors target naturally occurring non-productive alternative splicing using antisense oligonucleotides to promote the production of functional proteins.
Kian Huat Lim, Zhou Han, Hyun Yong Jeon, Jacob Kach, Enxuan Jing, Sebastien Weyn-Vanhentenryck, Mikaela Downs, Anna Corrionero, Raymond Oh, Juergen Scharner, Aditya Venkatesh, Sophina Ji, Gene Liau, Barry Ticho, Huw Nash, Isabel Aznarez   +15 more
doaj   +1 more source

Chemistry, structure and function of approved oligonucleotide therapeutics

Nucleic Acids Research, 2023
Abstract Eighteen nucleic acid therapeutics have been approved for treatment of various diseases in the last 25 years. Their modes of action include antisense oligonucleotides (ASOs), splice-switching oligonucleotides (SSOs), RNA interference (RNAi) and an RNA aptamer against a protein. Among the diseases targeted by this new class of
Martin Egli, Muthiah Manoharan
openaire   +2 more sources