Results 31 to 40 of about 179,623 (257)

Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain

Molecular Therapy: Nucleic Acids, 2015
Applications of RNA interference for neuroscience research have been limited by a lack of simple and efficient methods to deliver oligonucleotides to primary neurons in culture and to the brain.
Julia F Alterman, Lauren M Hall, Andrew H Coles, Matthew R Hassler, Marie-Cecile Didiot, Kathryn Chase, Jasmin Abraham, Emily Sottosanti, Emily Johnson, Ellen Sapp, Maire F Osborn, Marian Difiglia, Neil Aronin, Anastasia Khvorova   +13 more
doaj   +1 more source

Synthesis and evaluation of the functional oligonucleotides-PEG conjugates [PDF]

Nucleic Acids Symposium Series, 2004
We have previously demonstrated that the phenylsulfide and phenylsulfoxide derivatives of 2-amino-6-vinylpurine exhibit efficient cross-linking with high selectivity towards cytidine through synchronous activation within duplex. In this study, we synthesized their conjugates with PEG for possible application to in vitro as well as in vivo antisense ...
Ali Md, Monsur, Fumi, Nagatsugi, Motoi, Oishi, Yukio, Nagasaki, Kazunari, Kataoka, Shigeki, Sasaki   +5 more
openaire   +2 more sources

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

Molecules, 2017
In this review, we summarize the recent advances in the use of pyrene-modified oligonucleotides as a platform for functional nucleic acid-based constructs.
Olga A. Krasheninina, Darya S. Novopashina, Evgeny K. Apartsin, Alya G. Venyaminova   +3 more
doaj   +1 more source

A Simple and Efficient Microfluidic System for Reverse Chemical Synthesis (5′-3′) of a Short-Chain Oligonucleotide Without Inert Atmosphere

Applied Sciences, 2019
Reverse DNA synthesis (5′-3′) plays diverse functional roles in cellular biology, biotechnology, and nanotechnology. However, current microfluidic systems for synthesizing single-stranded DNAs at a laboratory scale are limited.
Rahul Bhardwaj, Phan T. Tue, Manish Biyani, Yuzuru Takamura   +3 more
doaj   +1 more source

Truncated Amplification: A Method for High-Fidelity Template-Driven Nucleic Acid Amplification

BioTechniques, 2002
The error rate of conventional PCR is problematic when amplifying from single cells or amplifying segments for protein functional analysis by in vitro translation. We describe truncated amplification, a method for high-fidelity amplification in which DNA
Qiang Liu, Piotr Swiderski, Steve S. Sommer   +2 more
doaj   +1 more source

Automated in vitro selection to obtain functional oligonucleotides [PDF]

Nucleic Acids Symposium Series, 2000
In vitro selection or systematic evolution of ligand by exponential enrichment (SELEX) has been devised for the identification of high-affinity oligonucleotide aptamers to target molecules. However, the selection process is repetitive and time-consuming.
H, Zhang, A, Hamasaki, E, Toshiro, Y, Aoyama, Y, Ito   +4 more
openaire   +2 more sources

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Targeting transcription factors associated with hemoglobinopathies: Lessons from successful interventions and implications for cancer

Molecular Oncology, EarlyView.
This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu, Puspa Das, Xing Zhang, Xiaodong Cheng   +3 more
wiley   +1 more source

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Modified Nucleoside Triphosphates for in-vitro Selection Techniques

Frontiers in Chemistry, 2016
The development of SELEX (Selective Enhancement of Ligands by Exponential Enrichment) provides a powerful tool for the search of functional oligonucleotides with the ability to bind ligands with high affinity and selectivity (aptamers) and for the ...
Adolfo Marcelo Iribarren, Adolfo Marcelo Iribarren, María eDellafiore, Javier Marcelo Montserrat, Javier Marcelo Montserrat   +4 more
doaj   +1 more source