Results 71 to 80 of about 704,707 (168)

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

In Vitro Techniques to Domesticate Mortiño (<i>Vaccinium floribundum</i> Kunth) and Other <i>Vaccinium</i> Species: A Review. [PDF]

Plants (Basel)
Coronel Montesdeoca NT, Jácome Sarchi GA, Martínez R, Hernández F.   +3 more
europepmc   +1 more source

In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

FEBS Open Bio, EarlyView.
In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller, Jana Bleher, Birgit Weinberger, Lourdes Rocamora‐Reverte   +3 more
wiley   +1 more source

Application of preclinical absorption, distribution, metabolism, elimination in vitro techniques for the characterization and compound library optimization of novel antibiotic gallium salophen. [PDF]

Drug Metab Dispos
Krug SA, Frank A, Hwang L, Worth M, Johnson K, Rojas C, Muller L, Michel SLJ, Wilks A, Xue F, Kane MA.   +10 more
europepmc   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source

Discovering the Anti-Inflammatory Potential of Compounds Isolated from the Aerial Parts of <i>Gelasia tomentosa</i> (L.) Zaika, Sukhor. & N.Kilian (Syn. <i>Scorzonera tomentosa</i>), Through In Vitro Techniques and Advanced In Silico Modeling Approaches. [PDF]

Molecules
Bahadır-Acıkara Ö, Akcan B, Yılmaz-Sarıaltın S, Zidorn C.   +3 more
europepmc   +1 more source

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Fermentation characteristics and nutritional value of whole and defatted black soldier fly larvae meal for ruminants using <i>in vitro</i> techniques. [PDF]

Vet Anim Sci
Sheikhalipour A, Hosseinkhani A, Taghizadeh A, Khalilvandi-Behroozyar H, Ahmadzadeh-Gavahan L.   +4 more
europepmc   +1 more source

The Comprehensive Live Cell‐Based Cytotoxicity Assay for Monitoring Disease Activity and Guiding Rescue Therapy in Acute Attacks of NMOSD

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Neuromyelitis optica spectrum disorder (NMOSD) is a devastating neurological disease that lacks serological biomarkers that can accurately reflect disease activity. We established a live cell‐based assay (LCBA) using serum with endogenous complement to quantify the overall cytotoxicity, offering a novel functional tool for monitoring
Xiaona Xu, Jie Ding, Yupeng Du, Guibao Luo, Jinming Li, Rui Liu, Xiaoyi Xu, Xiao Li, Minshu Li, Wei Jiang, Chunsheng Yang   +10 more
wiley   +1 more source

Biotechnological progresses in modelling the human endometrium: the evolution of current in vitro techniques and emerging trends. [PDF]

Front Bioeng Biotechnol
Agustina-Hernández M, Francés-Herrero E, Gómez-Álvarez M, Alonso-Frías P, Romeu M, Monzó A, Ferrero H, Bueno-Fernandez C, Cervelló I.   +8 more
europepmc   +1 more source