Results 161 to 170 of about 1,790,703 (269)

Dysfunctional CD4 T cells in an oncovirus-specific TCR-transgenic in vivo model. [PDF]

Nat Commun
Spitzer FS, Camps MGM, Britten CM, Vloemans S, van Zeeburg HJT, Melief CJM, Arakelian T, Ossendorp F.   +7 more
europepmc   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

The accumulation of extracellular K⁺ as in vivo model of epilepsy in CA1 pyramidal neurons. [PDF]

Sci Rep
Carpentieri V, Bernard C, Migliore M.
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

A human ex vivo model of radiation-induced skin injury recapitulates p53-driven profibrotic response to radiotherapy. [PDF]

JCI Insight
Dodson C, Bilik SM, DiBartolomeo G, Pachalis H, Siegfried LG, Johnson JA, Thaller SR, Pastar I, Tomic-Canic M, Griswold AJ, Stone RC.   +10 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

An Ex Vivo Model of Intervertebral Disc Degeneration for Assessing Retention of Injectable Cell-Based Grafts. [PDF]

JOR Spine
Schmid R, Apte J, Schulze E, Sirek A, Schäfer G, Schäper J, Santini F, Negoias S, Barbero A, Martin I, Pelttari K, Schären S, Krupkova O, Mehrkens A.   +13 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Tripartite motif containing 21 autoimmunity primes CD8⁺ cell-mediated lung inflammation: an in vivo model of interstitial lung disease pathogenesis. [PDF]

Arthritis Res Ther
Qiu Y, Wu Q, Liu L, Zhao W, Wang Y, Wang F, Tan W.   +6 more
europepmc   +1 more source