An accessible HPLC-DAD method for the direct detection of acrolein-trapping compounds in complex plant matrices. [PDF]
Zedet A +6 more
europepmc +1 more source
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley +1 more source
A Rapid and Precise Method for Nitrate Determination using a Step-Flow Autoanalyzer with Online Stopover Vanadium Reduction. [PDF]
Pai SC, Chang SK, Chien CT, Ho TY.
europepmc +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Incubation Behaviour of a Captive Female Great Grey Owl (<i>Strix nebulosa</i>) for an Unsuccessful and a Successful Hatching. [PDF]
Kwieciński Z, Mikkola H.
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Research Note: Measuring the electrocardiogram of male and female chicken embryos using wireless telemetry. [PDF]
Bigge A, Purdum S, Hanford K.
europepmc +1 more source
Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley +1 more source
Correction: Dual-responsive nanoparticles targeting ACE-II senescence for therapeutic mitigation of acute lung injury. [PDF]
Gao L, Zheng F, Fu Z, Wang W.
europepmc +1 more source

