FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Increasing fire frequency and severity will increase habitat loss for a boreal forest indicator species. [PDF]
Ecol Appl, 2022 Palm EC +9 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
What evidence exists on the effect of the main European lowland crop and grassland management practices on biodiversity indicator species groups? A systematic map protocol. [PDF]
Environ Evid, 2022 Séchaud R +3 more
europepmc +1 more source
FEBS Open Bio, EarlyView.14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa +2 more
wiley +1 more source
The complete plastid genome of Terminalia myriocarpa Vaniot Huerck et Muell.-Arg (Combretaceae), a tropical rainforest indicator species in Southern China. [PDF]
Mitochondrial DNA B Resour, 2022 Yu X, Zhao ZN, Ping HL, Deng LL.
europepmc +1 more source
FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Evaluating elements of artificial cover object design for terrestrial salamander monitoring
Wildlife Society BulletinDeclines in amphibian populations have led to greater research and monitoring of species of interest, including terrestrial salamanders. Several methods have been used to study salamanders, including artificial cover objects (ACOs).
Alison E. Ochs +2 more
doaj +1 more source
Internalization, reduced growth, and behavioral effects following exposure to micro and nano tire particles in two estuarine indicator species. [PDF]
Chemosphere, 2022 Siddiqui S +7 more
europepmc +1 more source
FEBS Open Bio, EarlyView.5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley +1 more source