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Development and optimization of in-house made indirect ELISA kit for the detection of antibodies against Pasteurella multocida in chicken [PDF]
BMC ImmunologyFowl cholera is caused by P. multocida. The currently available commercial kit is relatively expensive for Ethiopian laboratories. To address this issue indirect ELISA kit to detect antibodies against P. multocida in chicken was developed.
Dubale Beyene +2 more
doaj +2 more sources
Molecules, 2008 In this study novel immunoconjugates were designed, synthesized and then used to develop a rapid, specific and sensitive indirect ELISA method to directly detect residues of 3,5-dinitrosalicyclic acid hydrazide (DNSH), a toxic metabolite of nifursol ...
Hong Wang, Yudong Shen, Hongtao Lei
exaly +3 more sources
Recombinant MS087-based indirect ELISA for the diagnosis of Mycoplasma synoviae [PDF]
Frontiers in Veterinary ScienceAccurate detection is a prerequisite for effective prevention and control of Mycoplasma synoviae infection. ELISA is the most popular method for the clinical detection of M. synoviae because of its convenience, low cost, and high detection rate. However,
Yang Zhang +4 more
doaj +2 more sources
Preparation of a house ELISA kit for detecting Peste des petits ruminants Virus (PPRV) antibodies [PDF]
Benha Veterinary Medical Journal, 2021 Peste des petits ruminants (PPR) is a viral disease that affects small ruminants characterized by fever, highly contagious, and economically significant. For diagnosis of the disease, effective diagnostic techniques are essential.
Wafaa Hosny +3 more
doaj +1 more source
Study of prevalence of equine herpes virus types 1 and 4 infections in horse sera from riding clubs of Urmia [PDF]
Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī, 2023 Herpesviruses can cause significant economic losses to the equine industry worldwide. Equine herpes virus type 1 (EHV-1) and type 4 (EHV-4) cause respiratory signs, abortion, neonatal death and in some cases distal limb edema and myeloencephalopathy in ...
Amir Shafaroodi, Arash Araghi-Sooreh
doaj +1 more source
Establishment of an Indirect Competitive ELISA for Zearalenone
Shipin gongye ke-ji, 2022 In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method.
Song TANG +7 more
doaj +1 more source
Journal of Pure and Applied Microbiology, 2023 Brucellosis caused by various species of the genus Brucella is one of the most important zoonotic diseases of global importance with veterinary, public health, and economic concerns.
Sudipta Patra +7 more
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Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianae
Plant Protection Science, 1999 A monoclonal antibody (MAb 18) was prepared against purified mycelial proteins from Phytophthora nicotianae var. nicotianae. The specificity of MAb 18 (lgG class) was tested using indirect ELISA (PTA-ELISA).It cross-reacted with Phytophthora cacto rum ...
Blanka Pekárová-Kyněrová +1 more
doaj +1 more source
Animals, 2020 Haemonchus contortus is an important gastrointestinal nematode of small ruminants that causes significant mortality in goats worldwide. Diagnosis of this infection mainly depends on the evaluation of clinical signs and fecal examination.
Kalibixiati Aimulajiang +11 more
doaj +1 more source
A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis [PDF]
Veterinary World, 2019 Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats).
Marwa M. Attia +2 more
doaj +1 more source