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Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
Cold Spring Harbor Protocols, 2017The indirect competitive ELISA (indirect cELISA) pits plate-immobilized antigen against antigens in solution for binding to antigen-specific antibody. The antigens in solution are in the test sample and are first incubated with antigen-specific antibody.
Thomas O, Kohl, Carl A, Ascoli
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Enzyme-Based Methods for IgM Serology: Standard Indirect ELISA vs Antibody-Capture ELISA
Laboratory Medicine, 1992In a comparison of the performance (sensitivity and specificity) of standard indirect enzyme-linked immunosorbent assay (ELISA) and two antibody-capture ELISA methods, 50 serum specimens were tested for immunoglobulin M (IgM) to Toxoplasma gondii , and 54 serum specimens were tested for IgM to cytomegalovirus.
Albert J. Wilson +3 more
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AN INDIRECT COMPETITIVE ELISA FOR DETERMINATION OF CITRININ
Journal of Food Safety, 2011Citrinin (CIT)–protein artificial antigen was prepared by conjugating CIT with keyhole limpet hemocyanin by 1,4-butanediol diglycidyl ether. By immunization and fusion, a hybridoma cell line named K2-F3, which stably secreted the monoclonal antibody (McAb) against CIT was obtained.
YONGNING LI, YUANYUAN WANG, YANGHAO GUO
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2015
The enzyme-linked immunosorbent assay (ELISA) is a simple and rapid technique for detecting and quantitating antibodies or antigens attached to a solid surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries.
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The enzyme-linked immunosorbent assay (ELISA) is a simple and rapid technique for detecting and quantitating antibodies or antigens attached to a solid surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers, and quality control in various industries.
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An indirect sandwich ELISA for the identification of bovine enteroviruses
Journal of Virological Methods, 1993An indirect sandwich ELISA is described for the detection of bovine enteroviruses. The assay was developed as an alternative to the complement fixation test and proved to be more sensitive and convenient. Ten bovine enterovirus prototype strains were easily discriminated.
M C, Höfner +3 more
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Plant virus detection using a new form of indirect ELISA
Journal of Virological Methods, 1985A novel form of indirect enzyme-linked immunosorbent assay (ELISA) has been devised for the detection of viruses in plants. The method uses protein A in two applications to sandwich antibody-antigen-antibody layers. The first applied layer of protein A prepares the plate for the coating antibody layer. The second layer of protein A is conjugated to the
M L, Edwards, J I, Cooper
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A rapid method to improve protein detection by indirect ELISA
Biochemical and Biophysical Research Communications, 2011The enzyme-linked immunosorbant assay (ELISA) is a rapid, high-throughput, quantitative immunoassay for the selective detection of target antigens. The general principle behind an ELISA is antibody mediated capture and detection of an antigen with a measurable substrate.
Robert, Hnasko +3 more
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Indirect measurement of Epstein-Barr virus neutralising antibodies by ELISA
Journal of Virological Methods, 1998A rapid and effective ELISA for measuring Epstein-Barr virus (EBV)-neutralizing antibodies in human sera was devised to replace the existing cumbersome method involving the inhibition of fetal cord blood B-cell transformation by the virus. The new method will be invaluable for assessing antibody responses in human subjects participating in EBV gp340 ...
A D, Wilson, A J, Morgan
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Comparison of direct and indirect ELISA for detecting antigenically related cucumoviruses
Journal of Virological Methods, 1981The suitability of two ELISA procedures for detecting serologically closely related as well as distantly related cucumoviruses has been compared. When antibodies to a single strain were used, closely related strains of cucumber mosaic virus could be detected by both direct and indirect ELISA.
Devergne, J.C. +3 more
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Charting Methods for Internal Quality Control of Indirect ELISA
2009This chapter deals with control charts to monitor the performance of Indirect ELISAs. An Indirect ELISA kit for the detection of antibodies against Brucella is used to demonstrate the methods. Many of the features explained in Chapter 9 are relevant to this chapter; some repetition is intended, as this chapter may be read independently.
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