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Performance of Indirect ELISA for Maize Hybrid Purity Test
Advanced Materials Research, 2013Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid.
Yue Hui Liu +4 more
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Indirect ELISA for serosurveillance of Japanese encephalitis in pigs
Indian Journal of Animal Research, 2015For monitoring the activity of Japanese encephalitis (JE) in pigs, no effective assay is available in Indian context. Being its endemicity in India and role of pigs as amplifiers, there was need to develop rapid and sensitive assay for serosurveillance of JE in pigs.
R.P. Kolhe +5 more
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Uncertainty of measurement for competitive and indirect ELISAs.
Revue scientifique et technique (International Office of Epizootics), 2008A method for the estimation of the uncertainty of measurements for Gaussian outcomes of enzyme-linked immunosorbent assay (ELISA) is described using competitive and indirect foot and mouth disease (FMD) ELISAs. Assay repeatability was determined by random effects analysis of variance, and the normality of the residuals was checked.
J F, Toussaint +9 more
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Anti-dengue IgG detection by an indirect ELISA.
The Southeast Asian journal of tropical medicine and public health, 1997Protein-free culture media were originally developed for hybridomas to simplify downstream processing and purification. For the same reasons, we have used these protein-free media for passaging dengue 2 virus in C6/36 cells. This provided us with an infected supernatant (DenPF) which could then be used as coating antigens for an indirect enzyme-linked ...
P H, Tio, P, Malasit
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การประยุกต์ใช้เทคนิค indirect ELISA ในการวินิจฉัยโรคเมลิออยโดซิสในโลมาปากขวด (Tursiops truncatus)
การทดลองครั้งนี้ได้ทำการศึกษาเปรียบเทียบคุณสมบัติต่างๆ ของเชื้อ Burkholderia pseudomallei ที่แยกได้จากเลือดผู้ป่วยในประเทศไทย และเลือดโลมาปากขวดที่ป่วยในเกาะฮ่องกง เพื่อนำเทคนิค indirect ELISA ที่ถูกพัฒนาขึ้นเพื่อการวินิจฉัยโรคเมลิออยโดซิสในมนุษย์ นำมาประยุกต์ในโลมาปากขวด สิ่งที่ศึกษา ได้แก่คุณสมบัติทางชีวเคมีของเชื้อโดยใช้ API 20NE พบว่า เชื้อที่แยกได้openaire +1 more source
[Indirect ELISA for the pyrethroids-permethrin residue detection].
Huan jing ke xue= Huanjing kexue, 2007In order to detect the permethrin (Py) residue in environment samples, Ovalbumin(OVA)was used as protein carrier to couple with semi-antigen permethrin by active ester method. Indirect competitive ELISA (icELISA) was established. The most appropriate titration of coating antigen was 0.45 microg/mL and optimal dilution was 1/2 500 correspondingly.
Ting-feng, Liu, Ya-zi, Liu, Cheng, Sun
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Berliner und Munchener tierarztliche Wochenschrift, 2003
A total of 2754 bovine blood samples were examined for BHV1 antibodies in three different BHV-1 ELISA tests. The tests used were the CHEKIT Trachitest 2nd. Gen. "Bestätigungstest" Serum, the CHEKIT-BHV-1-gB-ELISA (Bommeli-Diagnostics/Intervet), and the BHV-1-gB Antibody Test Kit (IDEXX).
Gisela, Isa +2 more
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A total of 2754 bovine blood samples were examined for BHV1 antibodies in three different BHV-1 ELISA tests. The tests used were the CHEKIT Trachitest 2nd. Gen. "Bestätigungstest" Serum, the CHEKIT-BHV-1-gB-ELISA (Bommeli-Diagnostics/Intervet), and the BHV-1-gB Antibody Test Kit (IDEXX).
Gisela, Isa +2 more
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[Diagnosis of extraintestinal amebiasis by an indirect ELISA method].
Bulletin de la Societe de pathologie exotique et de ses filiales, 1984Immunoglobulins G and M, parenchymal lysis witness, are searched by Indirect ELISA technique. This methodology, epidemiologic studies applied, presents with reference technique: Indirect Hemagglutination a good correlation (r = 0.93). Other qualities are big specificity and good reproducibility .
P, Michel +3 more
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
exaly
[Diagnosis of trichinellosis in living pigs using indirect ELISA].
Berliner und Munchener tierarztliche Wochenschrift, 1995A modified E/S-ELISA based on the procedure described by Gamble et al. (1988) was used for the diagnosis of trichinellosis in the domestic pig. The results of screening the sera of 92 pigs experimentally infected with Trichinella larvae (T. spiralis, T. nativa) with different doses, confirmed that the E/S-ELISA is suitable for the serological detection
K, Nöckler +4 more
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