Expanding the Genetic Toolkit of Pichia kudriavzevii E1 for Enhanced Mycoprotein Production [PDF]
Microbial BiotechnologyPichia kudriavzevii is gaining attention as a non‐conventional yeast with significant industrial potential, owing to its robust tolerance to low pH and diverse process‐related stresses.
Yupeng Wang +7 more
doaj +3 more sources
Frontiers in Bioengineering and BiotechnologyWickerhamomyces ciferrii: a non-conventional yeast with significant industrial potential for tetraacetyl phytosphingosine (TAPS), remains underutilized due to the lack of a comprehensive genetic toolbox.
Seong-Rae Lee +2 more
doaj +4 more sources
Advancement in production engineering education through Virtual Learning Factory Toolkit concept [PDF]
Proceedings of the Estonian Academy of Sciences, 2021 The growing relevance of digitalization in production requires the enhancement of human skills and competences in the field of Information and Communication Technology (ICT).
Kashif Mahmood +9 more
doaj +2 more sources
A Toolkit for Effective and Successive Genome Engineering of Escherichia coli
Fermentation, 2022 The bacterium Escherichia coli has been well-justified as an effective workhorse for industrial applications. In this study, we developed a toolkit for flexible genome engineering of this microorganism, including site-specific insertion of heterologous ...
Bahareh Arab +3 more
doaj +2 more sources
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae
ACS Synthetic Biology, 2023 Synthetic biology toolkits are one of the core foundations on which the field has been built, facilitating and accelerating efforts to reprogram cells and organisms for diverse biotechnological applications.
Thomas Ellis +2 more
exaly +2 more sources
PYEAST - A Computational Toolkit for Saccharomyces cerevisiae Genetic Engineering. [PDF]
NPJ Syst Biol ApplSaccharomyces cerevisiae is a widely used biotechnological workhorse in both academic and industrial settings. One reason for its continued popularity is the extensive legacy of genetic tools, developed over its long history of use, that enable precise ...
Madika A +6 more
europepmc +2 more sources
Development and characterization of a Cre/<i>loxP</i> toolkit for genome engineering in <i>Komagataella phaffii</i>. [PDF]
Synth Syst BiotechnolThe Cre/loxP system is a versatile tool for genome engineering, yet its application in the industrial yeast Komagataella phaffii has been limited primarily to marker recycling. This study reports a comprehensive Cre/loxP toolkit for K. phaffii.
Song L +6 more
europepmc +2 more sources
Industrial Evaluation of a Toolkit of Methods for Engineering Knowledge Management of Simulations
Conference Proceedings of the Academy for Design Innovation Management, 2019 Simulations offer the potential of improving product and development process quality. However, a lack of overview of existing simulation methods and of documentation may make it difficult to apply them efficiently along the entire development process.
Nils MARAHRENS +3 more
openaire +2 more sources
A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12
Scientific Reports, 2020 Recent advances in CRISPR/Cas9 based genome editing have considerably advanced genetic engineering of industrial yeast strains. In this study, we report the construction and characterization of a toolkit for CRISPR activation and interference (CRISPRa/i)
Yvonne Nygård
exaly +2 more sources
Beyond Cutting: CRISPR-Driven Synthetic Biology Toolkit for Next-Generation Microalgal Metabolic Engineering. [PDF]
Int J Mol SciMicroalgae, with their unparalleled capabilities for sunlight-driven growth, CO2 fixation, and synthesis of diverse high-value compounds, represent sustainable cell factories for a circular bioeconomy.
Yang L, Lu Q.
europepmc +2 more sources