Results 41 to 50 of about 416,654 (265)

Innovative Design and Scalable Manufacturing of a High-Performance Globe Valve with Plug-Type Disc [PDF]

JES: Journal of Engineering Sciences
This study presents the design and development of an advanced industrial-grade globe valve for enhanced flow control, excellent sealing performance, and augmented longevity.
Mohammad Musfeq Iqfath, Sakib Mahmud, Md Mahadi Hasan   +2 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Multi-objective design optimisation of a delta coordinate measurement machine [PDF]

MATEC Web of Conferences
A systematic approach for multi-objective optimisation in machine design is presented and further demonstrated through a case study on a delta Coordinate Measurement Machine (CMM).
Louw Jakobus Murray, de Ronde Willis, Marais Stephen, Maweni Thabisa   +3 more
doaj   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Preparation of Sensitivity Test Antibiotic Discs Using Some Nanomaterials to Detect Resistance of Pseudomonas Aergenosa to Some Antibiotics

Iraqi Journal of Industrial Research, 2021
This study was conducted to synthesize bentonite nanoparticles and load them on different antibiotics to increase the effectiveness of the antibiotics or treat the resistance of Pseudomonas aeruginosa to some types of antibiotics in the laboratory.
Zainab J. Ghaneim, May M. Ismzil, Ilham Khalaf, Juan F. Mustafa, Shatha A. Mahmood, Alya M. Abdulaa   +5 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Experimental and Numerical Simulation Investigation on Deep Drawing Process of Inconel 718 with and without Intermediate Annealing Thermal Treatments

Applied Sciences, 2020
The aeronautical industry is moving from high-capacity large-airplane construction to low-capacity small-airplane construction. With the change in the production volume, there is a need for more efficient manufacturing processes, such as stamping/deep ...
Unai Ulibarri, Lander Galdos, Eneko Sáenz de Argandoña, Joseba Mendiguren   +3 more
doaj   +1 more source