SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
The effect of enterprise digital transformation on supply chain efficiency and its transmission mechanism. [PDF]
Zhen Y, Shang L.
europepmc +1 more source
Corporate Social Responsibility and Employee Recruiting in the Tourism Industry
Aini P. Karani, Jonathon G. Day
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Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
Dual dimension assessment and green driven model for high quality industrial development level in Yangtze River Delta. [PDF]
Shi X, Yang X, Liang W, Zheng L.
europepmc +1 more source
Modern aspects of occupational safety at meat industry enterprises.
Olga Evtushenko, A. Siryc
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We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
Correction to "Population-Based Cosmetics Exposure Parameters: Survey Approaches and Cosmetics Usage Results From Different Countries". [PDF]
europepmc +1 more source
Author Correction: Introduction of Cistanche phelypaea fatty acids as a new natural neurotrophic supplement by evaluating its effects in normal and Alzheimer's diseased rats. [PDF]
Aboubaker DH +4 more
europepmc +1 more source

