Results 141 to 150 of about 17,677 (181)

Simultaneous detection of influenza virus type B and influenza A virus subtypes H1N1, H3N2, and H5N1 using multiplex real-time RT-PCR

Applied Microbiology and Biotechnology, 2011
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong, Li, Jianxiong, Cheng, Xiaowen, Zhao, Cunyou, Wang, Ting, Lv, Xing, Wang, Xin, Wu, Chunli, Zhang, Renli, Cheng, Jinquan, Hong, Xue, Yu, Muhua   +11 more
openaire   +3 more sources

[Characterization of M2 gene of H3N2 subtype swine influenza virus].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2011
M2 protein of influenza A virus is encoded by a spliced mRNA derived from RNA segment 7 and plays an important role in influenza virus replication. It is also a target molecule of anti-virus drugs. We extracted the viral genome RNAs from MDCK cells infected with swine influenza A virus (SIV) H3N2 subtype and amplified the SIV M2 gene by reverse ...
Xiaodu, Wang, Peijun, Chen, Yang, Shen, Yafeng, Qiu, Xufang, Deng, Zixue, Shi, Lina, Peng, Jinyan, Luo, Chao, Liu, Zhiyong, Ma   +9 more
openaire   +1 more source

Comparative study of the hemagglutinin and neuraminidase genes of influenza A virus H3N2, H9N2, and H5N1 subtypes using bioinformatics techniques

Canadian Journal of Microbiology, 2007
To investigate the genomic patterns of influenza A virus subtypes, such as H3N2, H9N2, and H5N1, we collected 1842 sequences of the hemagglutinin and neuraminidase genes from the NCBI database and parsed them into 7 categories: accession number, host species, sampling year, country, subtype, gene name, and sequence.
Insung, Ahn, Hyeon S, Son
openaire   +2 more sources

[Rescue of H3N2 subtype swine influenza virus and substitution of hemagglutinin, neuraminidase].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2009
To study the mechanisms of trans-species transmission of influenza virus for developing novel vaccine of influenza in future.We rescued H3N2 subtype swine influenza virus strain A/Swine/Henan/S4/01 successfully by a plasmid-base reverse genetics. Eight gene segments were synthesized by reverse transcriptase-PCR and cloned into bidirection expression ...
Jinling, Du, Ming, Liu, Chunguo, Liu, Tao, Yang, Hongtao, Li   +4 more
openaire   +1 more source

[Cloning and characterization of M1 gene of H3N2 subtype swine influenza virus].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010
Influenza A virus matrix protein (M1) is encoded by a spliced mRNA derived from RNA segment 7 and plays an important role in the virus life cycle. In the present study, we extracted the viral genome RNAs from allantoic fluid of 9-day-old embryonated chicken eggs infected with swine influenza A virus (SIV) H3N2 subtype and amplified the SIV M1 gene by ...
Lin, Guo, Xiaodu, Wang, Qingwei, Liu, Yang, Shen, Yafeng, Qiu, Xiangdong, Li, Manlin, Luo, Zhiyong, Ma   +7 more
openaire   +1 more source

PREVALENCE OF HEMAGGLUTINATION INHIBITION ANTIBODY TO CURRENT STRAINS OF THE H3N2 AND H1N1 SUBTYPES OF INFLUENZA A VIRUS IN SERA COLLECTED FROM THE ELDERLY IN 1976

American Journal of Epidemiology, 1982
Sera were collected in 1976 from 182 individuals born between 1876-1935, who included patients in a large local nursing home in Orange County, California, and patients and staff at the University of California, Irvine Medical Center. Sera were treated with receptor-destroying enzyme and assayed for hemagglutination inhibition (HI) antibody to recent ...
M, Rekart, K, Rupnik, T C, Cesario, J G, Tilles   +3 more
openaire   +2 more sources

Development of a Subtype-Specific Diagnostic System for Influenza Virus H3N2 Using a Novel Virus-Based Systematic Evolution of Ligands by Exponential Enrichment (Viro-SELEX)

Journal of Biomedical Nanotechnology, 2019
Aptamers are oligonucleotide molecules that bind to specific target molecules generated by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have high future potential for use in diagnostics and therapeutics as molecular probes that recognize target molecules. To develop aptamers against a target protein using a SELEX process,
Junyoung, Kwon, Chandan, Narayan, Chonsaeng, Kim, Min Jung, Han, Meehyein, Kim, Sung Key, Jang   +5 more
openaire   +2 more sources

Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus A(H1N1), A(H1N1)2009, and A(H3N2) subtypes by melting point analysis

European Journal of Clinical Microbiology & Infectious Diseases, 2011
The high mutation rate of influenza virus, combined with the increasing worldwide use of influenza virus-specific drugs, allows the selection of viruses that are resistant to the currently available antiviral medications. Therefore, reliable tests for the rapid detection of drug-resistant influenza virus strains are required.
M, Redlberger-Fritz, S W, Aberle, R, Strassl, T, Popow-Kraupp   +3 more
openaire   +2 more sources

[Genome sequence analysis of an H3N2 subtype swine influenza virus isolated from Guangdong province in China].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2009
An H3N2 subtype swine influenza virus, A/Swine/ Guangdong/01/2005 (H3N2), was isolated from pigs with influenza-like signs in Guangdong province in 2005. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the gene segments for sequencing analysis.
Yan, Yao, Gui-Hong, Zhang, Wen-Jun, Liu, Tie-Qiao, Chen, Lei, Sun   +4 more
openaire   +1 more source

Optimization of the monoclonal antibody 3E1 through W32I mutation enhances antiviral efficacy against influenza virus subtypes H1N1 and H3N2

Antiviral Research
The influenza virus has caused a global pandemic with significant morbidity and mortality, underscoring the need to optimize antibodies for improved antiviral efficacy. The monoclonal antibody 3E1 effectively neutralizes Group 1 influenza subtypes, H1 and H5, by inhibiting acid-induced conformational changes of hemagglutinin (HA).
Shanshan Guan, Qingyu Wang, Jiaojiao Nie, Xin Yao, Kunpeng Xie, Weihao Zhao, Yaotian Chang, Lvzhou Zhu, Jiaru Hui, Tieyan Yin, Xiaopan Liu, Yingxin Zhang, Yaming Shan   +12 more
openaire   +2 more sources