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Antiviral Action of Methylated β-Lactoglobulin on the Human Influenza Virus A Subtype H3N2

Probiotics and Antimicrobial Proteins, 2010
Antiviral activity of methylated β-lactoglobulin (Met-BLG) against H3N2 infected into MDCK cell lines depended on concentration of Met-BLG, viral load, and duration of infection. IC50% of the hemagglutination activity for 1 and 0.2 MOI (multiplicity of infection) after 24 h of incubation at 37 °C in the presence of 5% CO2 were 20 ± 0.8 and 17 ± 0.7 μg ...
Sitohy, Mahmoud Zaki   +4 more
openaire   +3 more sources

A Recombinant Pseudorabies Virus Encoding the HA Gene From H3N2 Subtype Swine Influenza Virus Protects Mice From Virulent Challenge

Veterinary Immunology and Immunopathology, 2006
The hemagglutinin (HA) gene of A/Swine/Inner Mogolian/547/2001 (H3N2) swine influenza virus (SIV) was recombined into the genome of pseudorabies virus (PRV) Bartha-K61 vaccine strain, generating a recombinant PRV expressing the HA gene, designated as rPRV-HA.
Guangzhi Tong, Zhi-Jun Tian
exaly   +3 more sources

Protection Against a European H1N2 Swine Influenza Virus in Pigs Previously Infected With H1N1 And/Or H3N2 Subtypes

Vaccine, 2003
A novel swine influenza virus, H1N2, circulates in European swine populations together with H1N1 and H3N2 viruses. This study examines whether post-infection immunity to H1N1 and/or H3N2 viruses provides cross-protection against H1N2 infection. Pigs (n=51) were inoculated intranasally with either Sw/Belgium/1/98 (H1N1) or Sw/Flanders/1/98 (H3N2), or ...
Kristien van Reeth   +2 more
exaly   +3 more sources

Evolution of the nucleotide sequence of influenza virus RNA segment 7 during drift of the H3N2 subtype

Gene, 1983
The complete genetic information contained in the influenza virus RNA segment 7 of the A/Bangkok/1/79 (H3N2) strain has been cloned by in vitro synthesis of the complementary dsDNA and its insertion into plasmid pBR322. The nucleotide sequence of the viral RNA segment has been determined from the cDNA insert.
J, Ortín   +6 more
openaire   +2 more sources

Serosurvey for Influenza Virus Subtypes H3N8 and H3N2 Antibodies in Free-Ranging Canids in Pennsylvania, USA

Journal of Wildlife Diseases, 2019
Canine influenza virus (CIV) subtypes H3N8 and H3N2 are endemic among domestic dog ( Canis lupus familiaris ) populations in the northeastern US. Infection of free-ranging carnivores with influenza virus has been sporadically reported. Generalist mesocarnivores that exploit anthropogenic, peri-urban habitats share a wide interface with domestic dogs ...
Peter M, DiGeronimo   +4 more
openaire   +2 more sources

[In vitro study of antiviral activity of Myramistin against subtypes H3N2 and H5N1 of influenza virus].

Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic], 2009
In vitro antiviral effect of myramistin on influenza virus (MDCK cell culture) was studied. The drug showed significant dose-dependent antiviral activity against the virus. When used prophylactically (1 hour before exposure to the virus) in subtoxic doses, myramistin was effective in inhibiting replication of the influenza virus [strains A/Aichi/2/68 ...
A P, Agafonov   +8 more
openaire   +2 more sources

Multiplex RT-PCR assay for differentiating European swine influenza virus subtypes H1N1, H1N2 and H3N2

Journal of Virological Methods, 2012
In Europe, three major swine influenza viral (SIV) subtypes (H1N1, H1N2 and H3N2) have been isolated in pigs. Developing a test that is able to detect and identify the subtype of the circulating strain rapidly during an outbreak of respiratory disease in the pig population is of essential importance.
Chiara, Chiapponi   +4 more
openaire   +2 more sources

Amplification of the entire genome of influenza A virus H1N1 and H3N2 subtypes by reverse-transcription polymerase chain reaction

Journal of Virological Methods, 2006
This study describes the development of a simple RT-PCR method to amplify the whole genome of the influenza A virus based on the amplification of full-length gene segments. Primers were designed based on the conserved regions of both the 5'-end and the 3'-end of each gene segment.
Chi-Ho, Chan   +7 more
openaire   +2 more sources

Simultaneous detection of influenza virus type B and influenza A virus subtypes H1N1, H3N2, and H5N1 using multiplex real-time RT-PCR

Applied Microbiology and Biotechnology, 2011
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza type B virus and influenza A virus subtypes H5N1, H3N2, and H1N1 has been described. The method exhibited a high specificity and sensitivity of approximately 10(1)-10(2) copies per microliter or 10(-3)-10(-2) TCID50/L for each
Fang, Shisong   +11 more
openaire   +3 more sources

[Characterization of M2 gene of H3N2 subtype swine influenza virus].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2011
M2 protein of influenza A virus is encoded by a spliced mRNA derived from RNA segment 7 and plays an important role in influenza virus replication. It is also a target molecule of anti-virus drugs. We extracted the viral genome RNAs from MDCK cells infected with swine influenza A virus (SIV) H3N2 subtype and amplified the SIV M2 gene by reverse ...
Xiaodu, Wang   +9 more
openaire   +1 more source

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