Results 271 to 280 of about 282,997 (350)

Expression of a constitutively active nitrate reductase increases SARS‐CoV‐2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence

Plant Biotechnology Journal, EarlyView.
Summary The production of coronavirus disease 2019 vaccines can be achieved by transient expression of the Spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus 2 in agroinfiltrated leaves of Nicotiana benthamiana, a process promoted by the co‐expression of viral silencing suppressor P19.
Louis‐Philippe Hamel, Marie‐Ève Paré, Francis Poirier‐Gravel, Rachel Tardif, Marc‐André Comeau, Pierre‐Olivier Lavoie, Andréane Langlois, Marie‐Claire Goulet, Marc‐André D'Aoust, Dominique Michaud   +9 more
wiley   +1 more source

Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
Summary In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high‐value peptides, and small molecules produced by heterologous enzymatic pathways.
Maxim D. Harding, Frank Sainsbury
wiley   +1 more source

Structural Design and Immunogenicity of a Novel Self‐Adjuvanting Mucosal Vaccine Candidate for SARS‐CoV‐2 Expressed in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT Mucosal vaccination for COVID‐19 to boost preexisting though insufficient systemic and local/mucosal immunity remains an attractive prospect but there are currently no licensed mucosal vaccines against this infection. Here, using a plant expression system, we developed a novel mucosal vaccine platform for respiratory viruses and demonstrated ...
Mi‐Young Kim, Andy Cano Tran, Ju Kim, Humblenoble Stembridge Ayuk, Adam Sparrow, Lorenzo Bossi, Megan Brown, Emil Joseph Vergara, Kathrin Göritzer, Elisabetta Groppelli, Tae‐Ho Kwon, Julian K. C. Ma, Yong‐Suk Jang, Rajko Reljic   +13 more
wiley   +1 more source

Reactogenicity of an Inactivated, Split-Virion Quadrivalent Influenza Vaccine in Infants and Children Aged ≥6 Months to <9 Years. [PDF]

Vaccines (Basel)
Nolan T, Albano FR, Oberije J, Piedrahita M, Hohenboken M.   +4 more
europepmc   +1 more source

Influenza vaccine—live

, 2013
Catherine J. Luke, Seema S. Lakdawala, Kanta Subbarao   +2 more
openaire   +1 more source

Nicotiana benthamiana as a Source of Cowpea Mosaic Virus‐Derived Particles That Specifically Package Designer RNAs

Plant Biotechnology Journal, EarlyView.
ABSTRACT The coupling of replication and encapsidation in the bipartite plant virus, cowpea mosaic virus (CPMV), has been used to specifically incorporate designer RNA molecules into viral particles via transient expression in Nicotiana benthamiana. This is achieved by placing the desired RNA sequence between the 5′ and 3′ UTRs of CPMV RNA‐2 so that ...
H. Peyret, S. N. Shah, Y. Meshcheriakova, J.‐W. Jung, K. Saunders, G. P. Lomonossoff   +5 more
wiley   +1 more source

Early lymph node T follicular helper cell signalling hub drives influenza vaccine response in an ancestrally diverse cohort. [PDF]

EBioMedicine
Siu JHY, Coelho S, Palomeras A, Belij-Rammerstorfer S, Barman N, Lee CH, Ströbel T, Thorpe CJ, Kaur C, Cole T, Remmert N, Fowler J, Pledger S, Dooley KB, Chan T, Höschler K, Zambon M, Opoka D, Szommer T, Kim SJ, Kumar V, Vanderslott S, Kaleebu P, Milicic A, Palmer DB, Lambe T, Marsden BD, Koohy H, Coles M, Dendrou CA, Pollock KM.   +30 more
europepmc   +1 more source

The Impacts of Environmental Factors and Culture Conditions on the Transient Expression of Recombinant Protein in Nicotiana benthamiana Under Controlled Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT I summarise and discuss the effects of environmental factors and culture conditions on the productivity of recombinant proteins, including biopharmaceuticals, in Nicotiana benthamiana via transient gene expression platforms. A primary focus is on controlled‐environment plant cultivation systems such as plant factories with artificial lighting.
Ryo Matsuda
wiley   +1 more source