Results 121 to 130 of about 1,271,926 (297)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Exploring information dissemination effect on social media: an empirical investigation. [PDF]
Yang B, Zhang R, Cheng X, Zhao C.
europepmc +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Information Dissemination in Random Networks
Die vorliegende Dissertation beschäftigt sich mit der Dissemination von Information in einem Kommunikationsnetzwerk mit Broadcast-Kanal. Die zentrale Frage, welcher wir uns in dieser Arbeit widmen, ist wie man eine Nachricht ausgehend von einem Quellknoten effizient an alle anderen Knoten im Netzwerk verteilt. Hierbei verfolgen wir zwei Hauptziele: (1)
openaire +3 more sources
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Retracted: Information Dissemination Model in Rural Live Broadcasting under Blockchain in the Era of Artificial Intelligence. [PDF]
Intelligence And Neuroscience C.
europepmc +1 more source
EOS Data and Information System (EOSDIS) [PDF]
In the past decade, science and technology have reached levels that permit assessments of global environmental change. Scientific success in understanding global environmental change depends on integration and management of numerous data sources.
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Dynamic Characteristics and Evolution Analysis of Information Dissemination Theme of Social Networks under Emergencies. [PDF]
Zhang Y, Xie Y, Shi V, Yin K.
europepmc +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source

