Results 111 to 120 of about 1,350,437 (285)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Study on the Effect of Sodium Silicate Solution Injection Timings on Electrochemical Reinforcement of Dredged Sludge

Buildings
To address the issue of uneven shear strength distribution in dredged sediment during electroosmosis treatment, a grouting system was employed to inject CaCl2 into the anode region and Na2SiO3 solution into the central region.
Jiangdong Lin, Mi Ai, Guohui Yuan, Long Wang, Ziyang Gao, Xiaobing Li, Hongtao Fu, Yongfei Fan   +7 more
doaj   +1 more source

Time To Inject Some Contrast into the Nephrotoxicity Debate

Kidney360
Iodinated contrast media have long been feared for causing contrast-induced AKI (CI-AKI), a concern rooted in early reports with high-osmolar agents. Experimental data suggest potential nephrotoxic mechanisms, yet clinical evidence from older uncontrolled studies was confounded by comorbidities and procedural risks.
Nans Florens, Julien Demiselle
openaire   +2 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Numerical Simulation Study on Combustion Characteristics of a Low-Speed Marine Engine Using Biodiesel

Journal of Marine Science and Engineering
The growth of global trade has fueled a booming shipping industry, but high pollutant emissions from low-speed marine diesel engines have become a global concern.
Guohe Jiang, Yuhao Yuan, Hao Guo, Gang Wu, Jiachen Chen, Yuanyuan Liu   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Effect of Injection Timing on Ammonia–Natural Gas Co-Combustion Performance of Marine Low-Speed Two-Stroke High-Pressure Direct Injection Engines

Energies
The development of a marine ammonia–natural gas co-combustion engine aims to achieve high power with low carbon and low nitrogen oxide (NOx) emissions. Using AVL Fire software, a numerical model of a marine dual-fuel engine with a large cylinder diameter
Shiyu Wang, Rongsheng Lin, Fubo Wang, Peng Zhang, Xinyue Liu, Namin Zhang, Yanjie Ma, Wenfeng Wu, Hongliang Yu   +8 more
doaj   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Powder injection moulding at Canada’s National Research Council

, 2008
The National Research Council is Canada's leading industrial R&D organisation and its Industrial Materials Institute, based in Boucherville, Québec, is a key centre in the ongoing development of powder injection moulding technology.
Powder Injection Moulding International Magazine
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