Results 61 to 70 of about 18,999,904 (305)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The complete chloroplast genome of Rosa cymosa (Rosaceae), a traditional medicinal plant in South China

Mitochondrial DNA. Part B. Resources, 2020
Rosa cymosa is a traditional medicinal and ornamental plant in China. Here, we report the complete chloroplast genome of R. cymosa. The chloroplast genome is 156,607 bp in length with 37.48% GC content, containing a small single-copy (SSC) region (18,763 
Mingyan Ding, Miao Liao, Peiqian Liu, Guoqing Tan, Yiqing Chen, Shi Shi   +5 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Development and validation of a high-throughput agglutination assay for O-serogrouping clinical Escherichia coli isolates

Microbiology Spectrum
Accurate O-serotyping of Escherichia coli remains essential for clinical surveillance, outbreak investigation, and evaluation of vaccines targeting invasive E. coli disease.
Ritu Shastry, Wenchi Shang, Sarah Janssen, Sarah Tete, Elizabeth Garcia Dominguez, Germie van den Dobbelsteen, Todd Davies, Brian Morrow   +7 more
doaj   +1 more source

The genesis of innovation and innovative development

Herald of Omsk University Series Economics
The article focuses on the origins of the theory of innovation and its development. Currently, the innovative development of regions, industries, and companies is becoming increasingly significant for accelerating economic growth and addressing restrictions imposed by other nations.
Vladimir Byvshev, Yulia Suslova, Andrey Voloshin, Ivan Pisarev   +3 more
openaire   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Characterization of the complete chloroplast genome of Rosa laevigata var. leiocarpus in China and phylogenetic relationships

Mitochondrial DNA. Part B. Resources, 2020
Rosa laevigata var. leiocarpus has excellent edible, ornamental and medicinal value in China. In this study, we presented the complete chloroplast genome of R. laevigata var. leiocarpus. The whole chloroplast genome is 156,373 bp in size, consisting of a
Zhiqian Sun, Shi Shi, Xingchen Pang, Jingfeng Chen, Yongbin Wu   +4 more
doaj   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Rapid identification of antibody impurities in size-based electrophoresis via CZE-MS generated spectral library

Scientific Reports
Methods for the reliable and effective detection and identification of impurities are crucial to ensure the quality and safety of biopharmaceutical products. Technical limitations constrain the accurate identification of individual impurity peaks by size-
Quan Liu, Jiaying Hong, Yukun Zhang, Qiuyue Wang, Qiangwei Xia, Michael D. Knierman, Jim Lau, Caleen Dayaratna, Benjamin Negron, Hirsh Nanda, Harsha P. Gunawardena   +10 more
doaj   +1 more source

Exploring Ghanaian medical students’ learning experiences during the COVID-19 lockdown: a case study of the University for Development Studies Medical School [version 2; peer review: 2 approved, 1 approved with reservations]

F1000Research
Background The COVID-19 pandemic has had a profound impact on health professions education, particularly in developing and middle-income countries. Despite the implementation of alternative educational strategies to facilitate remote learning while ...
Victor Mogre, Sophia Ewuenye Adwoa Kpebu, Bruce Ayabilla Abugri, Anthony Amalba, Bright Yammaha Amoore   +4 more
doaj   +1 more source