Results 101 to 110 of about 131,230 (261)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Pédagogies innovantes et classe inversée : un levier pour favoriser l’engagement des étudiants au supérieur ?

open access: yesRevue Hybrides
The development of innovative teaching methods and learner engagement is a subject of worldwide interest, and Morocco is no exception. Unlike the traditional method, active learning encourages student involvement, prompting teachers to rethink their ...
Jihane SBOUSSA
doaj  

Students bridging the diversity gap in dermatology—A mixed methods study of medical education

open access: yesJEADV Clinical Practice
Background After noticing a lack of diversity in pictures for skin conditions and dermatology teaching, seven medical students applied for funding from the University of Exeter to design a project with the aim of expanding their dermatology knowledge ...
Shihan Salihu   +3 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Relationship between Knowledge Sharing and Innovative Tendencies and Job Performance among High School Principals in Bandar Abbas [PDF]

open access: yesIranian Journal of Educational Research
Objective: In the present study the relationship between knowledge Sharing and innovative tendencies and job performance   among high school principals in Bandar Abbas. Methods: A descriptive survey was used for the study.
Mahnoosh Abedini
doaj  

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

open access: yesFEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento   +6 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Innovative survey methods for the digital documentation of vernacular architectural Heritage in Syria

open access: yes, 2009
In rural villages to the south of Aleppo (Siria), dome-shaped buildings made of adobe were studied in the Culture 2007 European research project Coupoles et Habitats. Une tradition constructive entre Orient et Occident: les villages de Syrie du Nord; these buildings represent an exceptionally important historical-architectural heritage.
TUCCI, GRAZIA   +2 more
openaire   +1 more source

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