Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Regional Input Output Table for the State of Punjab [PDF]
Because of policy relevance of regional input-output analysis, a vast literature on the construction of regional input-output tables has emerged in the recent past, especially on the non-survey and hybrid methods. Although, construction of regional input-
Singh, Inderjeet, Singh, Lakhwinder
core +1 more source
Dampak Kebijakan Input, Output, dan Perdagangan Beras terhadap Diversifikasi Pangan Pokok [PDF]
EnglishAs one of the five most populous countries in the world, Indonesia has a big challenge to meet the food needs of its people. Food diversification has long been an important agenda of the national agricultural development planning program, but the ...
Hartoyo, S. (Sri) +3 more
core
Why human connection is the true metric of research success
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan +3 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Properties of Eventually Positive Linear Input-Output Systems
In this paper, we consider the systems with trajectories originating in the nonnegative orthant becoming nonnegative after some finite time transient.
Sootla, Aivar
core
Distributed computing system with dual independent communications paths between computers and employing split tokens [PDF]
This is a distributed computing system providing flexible fault tolerance; ease of software design and concurrency specification; and dynamic balance of the loads.
Bolotin, Gary S. +4 more
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Analisis Input Output Provinsi Jawa Barat [PDF]
Jawa Barat Province has the rapid growth for industrial sector in 2001-2003. This sector will influence to another sector to make a simultaneous growth for the whole economy.
Hermawan, W. (Wawan)
core +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source

