Results 51 to 60 of about 595 (210)
FSTVAL: a new web tool to validate bulk flanking sequence tags
Plant Methods, 2012 Background Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking sequences obtained from transgenic
Kim Joung +11 more
doaj +1 more source
Light controls growth and development via a conserved pathway in the fungal kingdom. [PDF]
PLoS Biology, 2005 Light inhibits mating and haploid fruiting of the human fungal pathogen Cryptococcus neoformans, but the mechanisms involved were unknown. Two genes controlling light responses were discovered through candidate gene and insertional mutagenesis approaches.
Alexander Idnurm, Joseph Heitman
doaj +1 more source
Advanced Science, EarlyView.BioSenSRRF is an open‐source workflow that combines conventional FRET biosensors with SRRF reconstruction to generate sub‐diffraction FRET index maps on standard microscopes. The pipeline integrates automated image registration, SRRF reconstruction, quantitative FRET index calculation, and random line‐based hotspot analysis to uncover AURKA‐dependent ...
Nicolas Y. Jolivet +5 more
wiley +1 more source
Conditional ablation of the RFX4 isoform 1 transcription factor: Allele dosage effects on brain phenotype. [PDF]
PLoS ONE, 2018 Regulatory factor X4 (RFX4) isoform 1 is a recently discovered isoform of the winged helix transcription factor RFX4, which can bind to X-box consensus sequences that are enriched in the promoters of cilia-related genes.
Ping Xu +6 more
doaj +1 more source
Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function
Advanced Science, EarlyView.This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang +10 more
wiley +1 more source
Advanced Science, EarlyView.STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang +8 more
wiley +1 more source
A Graph Based Framework to Model Virus Integration Sites
Computational and Structural Biotechnology Journal, 2016 With next generation sequencing thousands of virus and viral vector integration genome targets are now under investigation to uncover specific integration preferences and to define clusters of integration, termed common integration sites (CIS), that may ...
Raffaele Fronza +3 more
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SDS‐CRISPR for Single‐Nucleotide Variant Detection
Advanced Science, EarlyView.Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan +12 more
wiley +1 more source
Early stages of leaf development in has mutant of Arabidopsis thaliana
Biologia Plantarum, 2011 The elucidation of molecular mechanisms underlying the leaf development can be facilitated by the detailed anatomical study of leaf development mutants.
D. Janošević +4 more
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Nature Communications, 2022 Epistasis can lead to different phenotypic consequences from the same mutation. Here the authors carry out a genome-wide analysis of conditionally essential genes in yeast, finding that gene essentiality changes tend to occur concordantly among ...
Piaopiao Chen +2 more
doaj +1 more source