Results 21 to 30 of about 2,240 (218)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

The ORP on-sky community access program for adaptive optics instrumentation development [PDF]

Adaptive Optics Systems VII, 2020
On-sky testing of new instrumentation concepts is required before they can be incorporated within facility-class instrumentation with certainty that they will work as expected within a real telescope environment. Increasingly, many of these concepts are not designed to work in seeing-limited conditions and require an upstream adaptive optics system for
Morris, Tim, Osborn, James, Reyes, Marcos, Montilla, Iciar, Rousset, Gérard, Gendron, Éric, Fusco, Thierry, Neichel, Benoit, Esposito, Simone, Garcia, Paulo, Kulcsár, Caroline, Correia, Carlos, Beuzit, Jean-Luc, Bharmal, Nazim, Bardou, Lisa, Staykov, Lazar, Bonaccini Calia, Domenico   +16 more
openaire   +2 more sources

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Suitability of GPUs for real-time control of large astronomical adaptive optics instruments [PDF]

Journal of Real-Time Image Processing, 2017
Adaptive optics (AO) is a technique for correcting aberrations introduced when light propagates through a medium, for example, the light from stars propagating through the turbulent atmosphere. The components of an AO instrument are: (1) a camera to record the aberrations, (2) a corrective mechanism to correct them, (3) a real-time controller (RTC ...
Urban Bitenc, Alastair G. Basden, Nigel A. Dipper, Richard M. Myers   +3 more
openaire   +3 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Disentangled cascade adaptive optics for the SPHERE instrument forthcoming upgrade

, 2023
The Spectro-Polarimetric High-contrast Exoplanet REsearch instrument (SPHERE) is a European Southern Observatory (ESO) exoplanet imaging instrument installed on the 8m Very Large Telescope at Paranal (Chile). The results obtained during 8 years of operation since its commissioning in 2014 have encouraged ESO to define new science objective and thus to ...
Galland, Nicolas, Raynaud, Henri Francois, Kasper, Markus, Goulas, Charles, Béchet, Clémentine, Ferreira, Florian, Tallon, Michel, Vidal, Fabrice, Langlois, Maud, Boccaletti, Anthony, Chauvin, Gael, Diolaiti, Emiliano, Gratton, Raffaele, Loupias, Magali, Milli, Julien, Wildi, François, Kulcsar, Caroline   +16 more
openaire   +2 more sources