Results 71 to 80 of about 178,578 (290)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Reinforced sol–gel thermal barrier coatings and their cyclic oxidation life [PDF]

, 2013
Cyclic oxidation life enhancement of sol–gel thermal barrier coatings is obtained via the reinforcement of the controlled micro-crack network that forms during the initial sintering of the deposit.
Ansart, Florence, Bonino, Jean-Pierre, Le Maoult, Yannick, Lours, Philippe, Pin, Lisa, Vidal, Vanessa   +5 more
core   +3 more sources

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Characterisation of microstructure, defect and high-cycle-fatigue behaviour in a stainless steel joint processed by brazing [PDF]

, 2019
We report the characterisation of microstructures and high-cycle-fatigue (HCF) properties of Type 304 stainless steel joints processed by brazing. Pure copper was applied as the filler metal for brazing at 1120 °C. A two-phase microstructure was obtained
Chen, B., et al., Jones, S., Li, Y., Parfitt, D., Zhang, X.   +5 more
core   +1 more source

Shear critical R/C columns under increasing axial load [PDF]

, 2020
Structural elements in old reinforced concrete (R/C) frame buildings are often prone to shear or flexure-shear failure, which can eventually lead to loss of axial load capacity of vertical elements and initiate vertical progressive collapse of a building.
Elwood, Ghannoum, Ghannoum, Izzuddin, Lew, Lynn, Murray, Ousalem, Sasani, Sezen, Sezen, Xu, Yu, Zimos, Zimos   +14 more
core   +2 more sources

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Heat Treated NiP–SiC Composite Coatings: Elaboration and Tribocorrosion Behaviour in NaCl Solution [PDF]

, 2009
Tribocorrosion behaviour of heat-treated NiP and NiP–SiC composite coatings was investigated in a 0.6 M NaCl solution. The tribocorrosion tests were performed in a linear sliding tribometer with an electrochemical cell interface.
A Hovestad, A Miranda, C. B. Santos, C. F. Malfatti, CF Malfatti, CF Malfatti, D Landolt, E Färber, F Assi, F Bigdeli, H. Hololeczek, H. M. Veit, HG Schenzel, I Apachitei, I Garcia, I Garcia, IR Aslanyan, IR Aslanyan, J Fransaer, J Takadoum, J.-P. Bonino, JP Bonino, JP Celis, JW Graydon, KH Hou, L Fedrizzi, M Kaisheva, M Verelst, M. Metzner, N Guglielmi, P Jemmely, P Jemmely, P Ponthiaux, Q Zhao, S Mischler, S Shawki, SL Kuo, YL Shi   +37 more
core   +2 more sources

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Size effect on the fracture resistance of rough and frictional cracks

Fracture and Structural Integrity, 2018
Elastic fracture mechanics commonly defines the fracture resistance of brittle materials within an idealised picture of planar and traction free cracks. An efficient approach to describe the interface conditions in real cracks, such as those occurring in
Andrea Spagnoli, Andrea Carpinteri, Michele Terzano   +2 more
doaj  

A Prediction Model for Concrete Cracks due to Chloride-Induced Corrosion

Advances in Materials Science and Engineering, 2020
The nonhomogeneity of concrete leads to randomness in the development and extension of cracks. Scholars have proposed different models to analyze the development of cracks. Different from existing works on crack development, in this paper, we establish a
Jiao Wang, Ling Li, X. G. Zhang, Yangang Zhao   +3 more
doaj   +1 more source