Results 71 to 80 of about 69,376 (248)

Insight into the evolution of voids in TiBw-reinforced titanium matrix composites during isothermal compression

Journal of Materials Research and Technology
The evolution of two distinct void types formed during isothermal compression of Ti55-TiBw composites in the α+β region was systematically investigated through quantitative analysis from various deformation parameters and deformation regions. The results
Zhiyuan Du, Jianhua Xie, Lian Li, Miaoquan Li   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Ultra‐Improved Interfacial Strength Between Metallic Surface and Polyurethane via Cost‐Effective Anodizing Process

Advanced Materials Interfaces
Interfacial strength at the metal‐adhesive joint is a pivotal parameter to define adhesive bond reliability and its overall mechanical performance. This study systematically investigates and optimizes sulfuric acid anodizing (SAA) parameters to promote ...
Umut Bakhbergen, Sherif Araby, Zhandos Tauanov, Nursultan Turdakyn, Reza Montazami, Fethi Abbassi, Gulnur Kalimuldina   +6 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Kinetics of Reverse Micelles Extraction of Nickel from Electroplating Wastewater Using Dinonylnaphthalene Sulfonic Acid

工程科学与技术, 2014
:The kinetics of Ni (Ⅱ) extraction by dinonylnaphthalene sulfonic acid (DNNSA) in kerosene from wastewater was investigated using a Lewis Cell. The different parameters affecting the extraction rate such as stirring speed, interfacial area，DNNSA ...
余静, 邱海浪, 夏雪, 江洪龙, 汪嘉杨, 叶芝祥, 杨迎春, 张雪乔   +7 more
doaj  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Local two-group bubble size model for adiabatic air–water flow in a large diameter pipe using CFD code

Case Studies in Thermal Engineering
Accurate prediction of bubble behavior in large diameter pipes is crucial for evaluating the performance of safety systems, steam generators, and heat exchangers in nuclear systems.
Jinyeong Bak, Manh Long Doan, Seongbae Park, Jae Jun Jeong, Byongjo Yun   +4 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source