Results 21 to 30 of about 248,522 (217)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Modeling Creep Fracture in Rock by Using Kelvin Discretized Virtual Internal Bond
Advances in Civil Engineering, 2018 Discretized virtual internal bond (DVIB) is a lattice model, which is composed of bond cells. Each bond cell has a finite number of bonds. The DVIB is used to model the creep fracture. It is done by introducing a viscous bond to the original hyperelastic
Wangyang He, Zhennan Zhang
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Propiedades de tableros aglomerados de partículas fabricados con madera de Eucalyptus urophylla
Maderas: Ciencia y Tecnología, 2017 Este es un estudio exploratorio para dar uso a la madera de Eucalyptus urophylla en la elaboración de tableros aglomerados de partículas. Por lo tanto, los objetivos fueron determinar sus propiedades físico-mecánicas y evaluar los resultados bajo norma ...
Leidig Rangel +3 more
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Brazilian Dental Science, 2021 Objective: The aim of this study was to evaluate the microshear bond strength of Universal adhesives to enamel and dentin after one week and eighteen months of water storage.
Rubens Nazareno Garcia +4 more
doaj +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Proton-Binding Motifs of Membrane-Bound Proteins: From Bacteriorhodopsin to Spike Protein S
Frontiers in Chemistry, 2021 Membrane-bound proteins that change protonation during function use specific protein groups to bind and transfer protons. Knowledge of the identity of the proton-binding groups is of paramount importance to decipher the reaction mechanism of the protein,
Ana-Nicoleta Bondar
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Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Maderas: Ciencia y Tecnología, 2018 This study shows that it is feasible to make one-layer experimental particleboards from banana chips. Mechanical properties were easily satisfied at lower dosing rates, however thickness swelling remains a problem. The addition of 1% wax to the boards
Antonios Papadopoulos
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