Results 171 to 180 of about 239,862 (283)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Free-Text Responses in a Nationally Representative Experimental Survey about End-of-Life Care Choices: ChatGPT-4o-Assisted Qualitative Analytical Study. [PDF]

JMIR Aging
Goldberg EM, Macis M, Bounds M, Picazo JG, Nicholas LH.   +4 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

National palliative care laws in Europe: do they enable access and effectively promote the development of palliative care within health systems? A comparative multimethod analysis. [PDF]

Eur J Public Health
Carpén TP, Monzón-Llamas L, Hannon BL, Pawłowski L, Saarto TH, Pöyhiä RT, Centeno C.   +6 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Healthcare conflict management in resource-constrained settings: evidence from two hospitals in Ghana. [PDF]

Hum Resour Health
Botchwey CO, Acquah-Greens J, Adutwumwaa S, Aggrey-Bluwey L, Opoku-Mensah FA, Nkpetri ES.   +5 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Educational Barriers and Facilitators Related to Iran's Pronatalist Population Policy: A Qualitative Study. [PDF]

Iran J Nurs Midwifery Res
Asadi L, Keshmiri F, Kohan S.
europepmc   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Venereological practice and the <i>Patronato de Protección a la Mujer</i> (patronage for the protection of women) in Catalonia during the Franco Dictatorship (1939-1975). [PDF]

Front Public Health
Grafl F, Steger F.
europepmc   +1 more source