Why Leave a Job Half Done? Recent Progress in Enzymatic Deracemizations [PDF]
Financial support of this work by the Spanish MICINN (Projects CTQ2011-24237 and CTQ2013-44153) is gratefully ...
Díaz Rodríguez, Alba+2 more
core +2 more sources
Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope [PDF]
We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a general expression for the fluorescence autocorrelation function that applies when those dynamics are linear.
arxiv +1 more source
ABSTRACT Combined cross‐linked enzyme aggregates (combi‐CLEAs) represent a promising carrier‐free immobilized enzyme technology. This study describes the preparation of combi‐CLEAs comprising leucine dehydrogenase (LeuDH) and formate dehydrogenase (FDH) for the regeneration of cofactor nicotinamide adenine dinucleotide necessary for 2‐aminobutyric acid
Jingran Liu+8 more
wiley +1 more source
Disulfides as redox switches : from molecular mechanisms to functional significance
The molecular mechanisms underlying thiol-based redox control are poorly defined. Disulfide bonds between Cys residues are commonly thought to confer extra rigidity and stability to their resident protein, forming a type of proteinaceous spot weld. Redox
Fan, Samuel W.+2 more
core +1 more source
Structure and Function of Bacillus subtilis YphP, a Prokaryotic Disulfide Isomerase with a CXC Catalytic Motif†,‡ [PDF]
: The DUF1094 family contains over 100 bacterial proteins, all containing a conserved CXCmotif, with unknown function. We solved the crystal structure of the Bacillus subtilis representative, the product of the yphP gene.
Andrzej Joachimiak+9 more
core +2 more sources
Banana skin: a novel material for a low-cost production of laccase [PDF]
Laccases (benzenodiol: oxygen oxidoreductases; EC 1.10.3.2) are multicopper oxidases of wide substrate specificity mainly found in white-rot fungi, which are the only microorganisms able to degrade the whole wood components, but they are also expressed in bacteria and higher plants. Laccases are used currently in biotechnological processes because this
arxiv
Short to long-range charge-transfer excitations in the zincbacteriochlorin-bacteriochlorin complex: a Bethe-Salpeter study [PDF]
We study using the Bethe-Salpeter formalism the excitation energies of the zincbacteriochlorinbacteriochlorin dyad, a paradigmatic photosynthetic complex. In great contrast with standard timedependent density functional theory calculations with (semi)local kernels, charge transfer excitations are correctly located above the intramolecular Q-bands ...
arxiv +1 more source
Folding of a single domain protein entering the endoplasmic reticulum precedes disulfide formation [PDF]
The relationship between protein synthesis, folding and disulfide formation within the endoplasmic reticulum (ER) is poorly understood. Previous studies have suggested pre-existing disulfide links are absolutely required to allow protein folding and ...
Bulleid, Neil J.+3 more
core +1 more source
Multi-scale Molecular Simulations on Respiratory Complex I [PDF]
Complex I (NADH:ubiquinone oxidoreductase) is a redox-driven proton pump that powers synthesis of adenosine triphosphate (ATP) and active transport in most organisms. This gigantic enzyme reduces quinone (Q) to quinol (QH2) in its hydrophilic domain, and transduces the released free energy into pumping of protons across its membrane domain, up to ca ...
arxiv
Richard Nelson Perham. 27 April 1937—14 February 2015 [PDF]
Richard Nelson Perham, FRS, FMedSci, FRSA, was a British professor of structural biochemistry. He undertook his academic career at the University of Cambridge, holding positions as lecturer, reader, chair and head of the Department of Biochemistry, as ...
Charoy, François+6 more
core +2 more sources