Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Plant-soil feedbacks do not explain invasion success of Acacia species in introduced range populations in Australia [PDF]
Legumes, especially acacias, are considered amongst the most successful invaders globally. However there is still very little known about the role of soil microbial communities in their invasion success in novel ranges.
Leishman, M.R. +2 more
core +1 more source
Relative influence of inter- and intraspecific competition in an ungulate assemblage modified by introduced species. [PDF]
Zini V, Wäber K, Dolman PM.
europepmc +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
A Case Study of the Population Ecology of a Topmouth Gudgeon (Pseudorasbora parva) Population in the UK and the Implication for Native Fish Communities [PDF]
1. The topmouth gudgeon Pseudorasbora parva is a small Asian cyprinid species that has proved invasive throughout many European countries. Following an initial introduction into the wild in 1996, the species is now proving invasive in the UK, with at ...
Brazier, Matt +3 more
core +1 more source
The alien spider species Spermophora kerinci Huber, 2005, Nesticella mogera (Yaginuma, 1972) and Pseudanapis aloha Forster, 1959 are recorded from greenhouses in Germany. These are the first records from the European mainland.
Kielhorn, Karl-Hinrich
doaj +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Introduced species shape insular mutualistic networks. [PDF]
Hui C.
europepmc +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Pollination ecology of the invasive tree tobacco Nicotiana glauca: comparisons across native and non-native ranges [PDF]
Interactions with pollinators are thought to play a significant role in determining whether plant species become invasive, and ecologically generalised species are predicted to be more likely to invade than more specialised species.
Ollerton, Jeff +30 more
core

