Expression, purification and characterisation of recombinant peptide:N-glycosidase F. : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Biochemistry at Massey University [PDF]
, 2000 PNGase F (Peptide-N4-(N-acetyl-D-glucosaminyl) asparagine amidase F) is an amidohydrolase isolated from the extracellular medium of the Gram-negative bacterium Flavobacterium meningosepticum.
Loo, Trevor Stephen
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Chronic Hypoxia Disrupts Spermatogenesis Through ASXL2–EZH2–Mediated Microtubule Destabilization
Advanced Science, EarlyView.This study reveals the mechanism by which chronic hypoxia impairs spermatogenesis via the ASXL2–EZH2 axis, hindering the transition of spermatids from round to elongated forms. Key findings reveal that under hypoxic conditions, downregulated ASXL2 expression reduces EZH2 binding to the CEP162 promoter, leading to decreased H3K27me3 modification and ...
Jun Yin +11 more
wiley +1 more source
A Bifunctional T3SS‐Effector Simultaneously Cleaves Host MAP Kinase and Inhibits PPM1A Phosphatase
Advanced Science, EarlyView.Pathogenic bacteria exploit the metalloprotease effector NleD to subvert host defenses. Structural, biochemical, and infection analyses reveal a bifunctional mechanism by which NleD binds and inhibits the host phosphatase PPM1A while preserving its proteolytic activity against MAPKs.
Yaakov Socol +18 more
wiley +1 more source
The conversion of L-histidine to glutamic acid by liver enzymes [PDF]
, 1952 Edlbacher and Neber (1) showed in 1934 that the liver enzyme named histidase degrades histidine to NH3, formic acid, and an unknown product which on further treatment with strong alkali yields glutamic acid.
Abrams, Adolph, Borsook, Henry
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Advanced Science, EarlyView.Time‐resolved SAXS reveals how physiologically relevant metal ions shape the earliest conformational responses of monomeric α‐synuclein. Fe3+ induces rapid compaction, Cu2+ generates heterogeneous and partially folded ensembles, while Mn2+ and Zn2+ cause only modest changes.
Rebecca Sternke‐Hoffmann +3 more
wiley +1 more source
Ion-Exchange Chromatography Coupled to Mass Spectrometry in Life Science, Environmental, and Medical Research. [PDF]
Anal Chem, 2023 Ngere JB +5 more
europepmc +1 more source
A Simplified and Selective Technique for The Direct Recovery of Glucose-6-Phosphate Dehydrogenase From Unclarified Yeast Feedstock [PDF]
, 2005 The development of a simplified and rapid technique for the selective recovery of proteins from Bakers' yeast was undertaken. Purification of Glucose-6-phosphate dehydrogenase (G6PDH) from Saccharomyces cerevisiae was chosen demonstrate of this ...
Chow, Yen Mei
core
Advanced Science, EarlyView.Phospholipid transfer protein(PLTP) plays a critical role in forming a complex with kinase A (AURKA) and P65. This interaction facilitates phosphorylation of P65 at Ser536, leading to the activation of the NF‐κB signaling pathway. Ultimately, this leads to the upregulation of downstream cytokines, including IL‐6, IL‐8, and CSF‐1, which promotes M2 ...
Xinyue Liang +14 more
wiley +1 more source
QCL-IR Spectroscopy for In-Line Monitoring of Proteins from Preparative Ion-Exchange Chromatography. [PDF]
Anal Chem, 2022 Akhgar CK +4 more
europepmc +1 more source
Endocytic Control of Cell‐Autonomous and Non‐Cell‐Autonomous Functions of p53
Advanced Science, EarlyView.NUMB Ex3‐containing isoforms localize to the plasma membrane, where they recruit p53 through SNX9 and direct it to multivesicular bodies and exosomes. Exported p53 is taken up by neighboring cells and activates nuclear programs, revealing an intercellular, exosome‐based pathway that might help establish a tumor‐suppressive microenvironment.
Roberta Cacciatore +20 more
wiley +1 more source