Ion-exchange chromatography purification of extracellular vesicles
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović+4 more
doaj +1 more source
Antimicrobial activity of endophytic fungi Kabatiella caulivora Var. A from Alyxia reinwardtii Bl.
Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7.
Y. Sri Wulan Manuhara
doaj +1 more source
Study on separation and purification process of C1 esterase inhibitor
Objective To study the technology of separating and purifying C1 esterase inhibitor (C1-INH) by using the waste washing liquid as raw materia during the preparation of human prothrombin complex (PCC) l.
Jiabin XU+4 more
doaj +1 more source
An ISFET-based anion sensor for the potentiometric detection of organic acids in liquid chromatography [PDF]
An ion-selective field effect transistor (ISFET) was applied as a potentiometric detector in liquid chromatography (LC) for the determination of organic acids.
Berg, A. van den+6 more
core +2 more sources
Identification of novel components of Trypanosoma brucei editosomes [PDF]
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole+6 more
core +2 more sources
Water, electricity and ion exchange; how Hamish Small sustained the evolution of ion chromatography
Since the development of synthetic ion exchange resins in 1935, the electrochemical properties of these unique materials have intrigued researchers. In 1951, Hamish Small was introduced to synthetic ion exchange resin when he studied the electromigration
John M. Riviello
doaj
Removal of disinfection by-product precursors by coagulation and an innovative suspended ion exchange process [PDF]
This investigation aimed to compare the disinfection by-product formation potentials (DBPFPs) of three UK surface waters (1 upland reservoir and 2 lowland rivers) with differing characteristics treated by (a) a full scale conventional process and (b ...
Jarvis, Peter+4 more
core +1 more source
Purification and Characterization of a Chloroplast Outer-Envelope-Bound, ATP-Dependent Protein Kinase [PDF]
An ATP-dependent protein kinase was partially purified from isolated outer envelope membranes of pea (Pisum sativum L., Progress No. 9) chloroplasts. The purified kinase had a molecular weight of 70 kilodaltons, as determined by sodium dodecyl sulfate ...
Soll, Jürgen
core +2 more sources
Silent hemoglobin variant during capillary electrophoresis: A case report
Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes.
Yanping Yuan+4 more
doaj +1 more source
In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column derivatization ...
Rafea Naffa, Joseph Pesek
doaj +1 more source