Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration.
Jelena Ruscic +4 more
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Total plasma homocysteine measurement: Evaluation of the Abbott immunoassay, comparison with the JEOL ion exchange chromatography and investigation of its clinical utility [PDF]
Practical Laboratory Medicine, 2022 Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen +6 more
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Ion-exchange chromatography purification of extracellular vesicles
BioTechniques, 2017 Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović +4 more
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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline. [PDF]
J Vis Exp, 2017 Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules.
Brennich ME, Round AR, Hutin S.
europepmc +2 more sources
Online ion-exchange chromatography for small-angle X-ray scattering. [PDF]
Acta Crystallogr D Struct Biol, 2016 SAXS coupled with online ion-exchange chromatography allows the collection of high-quality BioSAXS data.
Hutin S, Brennich M, Maillot B, Round A.
europepmc +2 more sources
Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]
BiomedicinesBackground: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
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Ion exchange membranes in ion chromatography and related applications.
Talanta, 2019 This review is a broad survey of ion exchange membranes (IEMs) in chosen areas of analytical chemistry. The coverage is comprehensive in ion chromatography (IC) applications, this includes in-line water purification for IC use, chemical and ...
P. Dasgupta, F. Maleki
semanticscholar +3 more sources
Hydrophobicity of polymer based anion-exchange columns for ion chromatography
Heliyon, 2021 The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers.
Pavel N. Nesterenko +1 more
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Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography.
J Chromatogr A, 2015 Highlights • Optimised chemistry protocols of DEAE and COO functionalisations.• Compression applied after electrospinning improved mechanical strength.• Increasing compression and bed layers lowered binding capacities.
Dods SR +3 more
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PURIFICATION AND PARTIAL CHARACTERIZATION OF SERUM IMMUNOGLOBULIN FROM PERSIAN STURGEON (ACIPENSER PERSICUS) [PDF]
Majallah-i ̒Ilmī-i Shīlāt-i Īrān, 2003 In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel ...
M. Kalbasi1; A. Mostafaei2; J. Majidi3
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