Results 11 to 20 of about 3,527,182 (275)

Characterization of Complex Proteoform Mixtures by Online Nanoflow Ion-Exchange Chromatography-Native Mass Spectrometry. [PDF]

open access: yesAnal Chem
The characterization of proteins and complexes in biological systems is essential to establish their critical properties and to understand their unique functions in a plethora of bioprocesses.
Zhai Z   +5 more
europepmc   +4 more sources

Ion-exchange membrane chromatography

open access: yes, 2023
Chromatography is a powerful separation technique that has become an important unit operation when high levels of purity are required. The selectivity of the separation process depends on the interactions between the stationary phase and the mobile phase
Boi C.   +4 more
core   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

open access: yesBioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović   +4 more
doaj   +1 more source

Antimicrobial activity of endophytic fungi Kabatiella caulivora Var. A from Alyxia reinwardtii Bl.

open access: yesJournal of Biological Researches, 2012
Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7.
Y. Sri Wulan Manuhara
doaj   +1 more source

Ion exchange chromatography protocol.

open access: yes, 2023
Schematic and summarized protocol for the isolation of sEVs from conditioned media by ion exchange chromatography.
Natalia Pacienza (4669699)   +5 more
core   +1 more source

Study on separation and purification process of C1 esterase inhibitor

open access: yesZhongguo shuxue zazhi, 2022
Objective To study the technology of separating and purifying C1 esterase inhibitor (C1-INH) by using the waste washing liquid as raw materia during the preparation of human prothrombin complex (PCC) l.
Jiabin XU   +4 more
doaj   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

open access: yes, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Dowling, Oonagh   +2 more
core   +2 more sources

ION EXCHANGE CHROMATOGRAPHY

open access: yes, 2023
Ion exchange chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. Ion exchange chromatography is probably the most powerful and classic type of liquid chromatography. It can also used for almost all kinds of charged molecule including large proteins, small nucleotides and amino
  +4 more sources

Silent hemoglobin variant during capillary electrophoresis: A case report

open access: yesJournal of Diabetes Investigation, 2020
Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes.
Yanping Yuan   +4 more
doaj   +1 more source

Development and characterisation of monolithic silica stationary phases for ion chromatography [PDF]

open access: yes, 2006
Silica based monolithic HPLC columns have received much attention in recent years, particularly those modified for reversed-phase separations. However, for ionexchange, silica based monolithic columns have received only limited attention.
Sugrue, Edel
core   +1 more source

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