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AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
A, Williams, V, Frasca
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Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois, Jungbauer, Rainer, Hahn
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Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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